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Literature summary extracted from

  • Livera, W.C.D.; Shimizu, C.
    Comparison and characterization of arginine kinases purified from the prawn Penaeus japonicus (Kurumaebi) and the swimming crab Portunus trituberculatus (1989), Agric. Biol. Chem., 53, 2377-2386.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.3.3 more stable in 100 mM Tris-HCl buffer at pH 8.0 than in 100 mM phosphate buffer at pH 7.0 Penaeus japonicus
2.7.3.3 more stable in 100 mM Tris-HCl buffer at pH 8.0 than in 100 mM phosphate buffer at pH 7.0 Portunus trituberculatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.3 Ca2+ 4 mM, activation Penaeus japonicus
2.7.3.3 Ca2+ 4 mM, activation Portunus trituberculatus
2.7.3.3 Co2+ 4 mM, activation Penaeus japonicus
2.7.3.3 Co2+ 4 mM, activation Portunus trituberculatus
2.7.3.3 Fe2+ 4 mM, activation Penaeus japonicus
2.7.3.3 Fe2+ 4 mM, activation Portunus trituberculatus
2.7.3.3 Mg2+ 4 mM, activation Penaeus japonicus
2.7.3.3 Mg2+ 4 mM, activation Portunus trituberculatus
2.7.3.3 Mg2+ highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP Penaeus japonicus
2.7.3.3 Mg2+ highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP Portunus trituberculatus
2.7.3.3 Mg2+ highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate Penaeus japonicus
2.7.3.3 Mg2+ highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate Portunus trituberculatus
2.7.3.3 Mn2+ less effective than Mg2+ in activation Penaeus japonicus
2.7.3.3 Mn2+ less effective than Mg2+ in activation Portunus trituberculatus
2.7.3.3 Mn2+ 4 mM, activation Penaeus japonicus
2.7.3.3 Mn2+ 4 mM, activation Portunus trituberculatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.3 40000
-
 x * 40000, SDS-PAGE Portunus trituberculatus
2.7.3.3 40500
-
 x * 40500, SDS-PAGE Portunus trituberculatus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.3 Penaeus japonicus
-
-
-
2.7.3.3 Portunus trituberculatus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.3
-
 Penaeus japonicus
2.7.3.3
-
 Portunus trituberculatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.3 234
-
-
 Portunus trituberculatus
2.7.3.3 318
-
-
 Penaeus japonicus

Storage Stability

EC Number Storage Stability Organism
2.7.3.3 -20°C, 10 mM Tris-HCl buffer, pH 8.5, activity gradually decreases, but can be restored by addition of 1 mM 2-mercaptoethanol Penaeus japonicus
2.7.3.3 -20°C, 10 mM Tris-HCl buffer, pH 8.5, activity gradually decreases, but can be restored by addition of 1 mM 2-mercaptoethanol Portunus trituberculatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.3 ATP + L-Arg
-
 Penaeus japonicus ADP + Nomega-phospho-L-Arg
-
 r
2.7.3.3 ATP + L-Arg
-
 Portunus trituberculatus ADP + Nomega-phospho-L-Arg
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.3 ?
-
 Penaeus japonicus
2.7.3.3 ? x * 40000, SDS-PAGE Portunus trituberculatus
2.7.3.3 ? x * 40500, SDS-PAGE Portunus trituberculatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.3.3 40
-
-
 Portunus trituberculatus
2.7.3.3 42
-
-
 Penaeus japonicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.3.3 10 60 10°C: about 50% of maximal activity, 60°C: about 60% of maximal activity Penaeus japonicus
2.7.3.3 10 60 10°C: about 40% of maximal activity, 60°C: about 40% of maximal activity Portunus trituberculatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.3.3 20
-
 10 min, stable up to Penaeus japonicus
2.7.3.3 20
-
 10 min, stable up to Portunus trituberculatus
2.7.3.3 25
-
 10 min, inactivation above Penaeus japonicus
2.7.3.3 25
-
 10 min, inactivation above Portunus trituberculatus
2.7.3.3 40
-
 10 min, 100 mM Tris-HCl buffer, pH 8.0, 34% loss of activity. 10 min, 100 mM Tris-HCl buffer, pH 7.0, 46% loss of activity Portunus trituberculatus
2.7.3.3 40
-
 10 min, 100 mM Tris-HCl buffer, pH 8.0, 15% loss of activity. 10 min, 100 mM Tris-HCl buffer, pH 7.0, 16% loss of activity Penaeus japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.3 5.8
-
 synthesis of ATP Portunus trituberculatus
2.7.3.3 6.1
-
 synthesis of ATP Penaeus japonicus
2.7.3.3 9
-
 synthesis of N-phospho-L-arginine Penaeus japonicus
2.7.3.3 9.2
-
 synthesis of N-phospho-L-arginine Portunus trituberculatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.3.3 5.5 8 pH 5.5: about 90% of maximal activity, pH 8.0: about 70% of maximal activity, no activity at pH 5.0, synthesis of ATP Penaeus japonicus
2.7.3.3 5.5 8 pH 5.5: about 90% of maximal activity, pH 8.0: about 70% of maximal activity, no activity at pH 5.0, synthesis of ATP Portunus trituberculatus
2.7.3.3 8 10.5 pH 8.0: about 60% of maximal activity, pH 10.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg Penaeus japonicus
2.7.3.3 8 10.5 pH 8.0: about 60% of maximal activity, pH 10.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg Portunus trituberculatus