BRENDA - Enzyme Database

Comparison and characterization of arginine kinases purified from the prawn Penaeus japonicus (Kurumaebi) and the swimming crab Portunus trituberculatus

Livera, W.C.D.; Shimizu, C.; Agric. Biol. Chem. 53, 2377-2386 (1989)
No PubMed abstract available

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.3.3
more stable in 100 mM Tris-HCl buffer at pH 8.0 than in 100 mM phosphate buffer at pH 7.0
Penaeus japonicus
2.7.3.3
more stable in 100 mM Tris-HCl buffer at pH 8.0 than in 100 mM phosphate buffer at pH 7.0
Portunus trituberculatus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
4 mM, activation
Penaeus japonicus
2.7.3.3
Ca2+
4 mM, activation
Portunus trituberculatus
2.7.3.3
Co2+
4 mM, activation
Penaeus japonicus
2.7.3.3
Co2+
4 mM, activation
Portunus trituberculatus
2.7.3.3
Fe2+
4 mM, activation
Penaeus japonicus
2.7.3.3
Fe2+
4 mM, activation
Portunus trituberculatus
2.7.3.3
Mg2+
4 mM, activation; highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP
Penaeus japonicus
2.7.3.3
Mg2+
4 mM, activation; highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP
Portunus trituberculatus
2.7.3.3
Mn2+
4 mM, activation; less effective than Mg2+ in activation
Penaeus japonicus
2.7.3.3
Mn2+
4 mM, activation; less effective than Mg2+ in activation
Portunus trituberculatus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
x * 40000, SDS-PAGE
Portunus trituberculatus
2.7.3.3
40500
-
x * 40500, SDS-PAGE
Portunus trituberculatus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.3
Penaeus japonicus
-
-
-
2.7.3.3
Portunus trituberculatus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.3
-
Penaeus japonicus
2.7.3.3
-
Portunus trituberculatus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.3.3
234
-
-
Portunus trituberculatus
2.7.3.3
318
-
-
Penaeus japonicus
Storage Stability
EC Number
Storage Stability
Organism
2.7.3.3
-20°C, 10 mM Tris-HCl buffer, pH 8.5, activity gradually decreases, but can be restored by addition of 1 mM 2-mercaptoethanol
Penaeus japonicus
2.7.3.3
-20°C, 10 mM Tris-HCl buffer, pH 8.5, activity gradually decreases, but can be restored by addition of 1 mM 2-mercaptoethanol
Portunus trituberculatus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642448
Penaeus japonicus
ADP + Nomega-phospho-L-Arg
-
642448
Penaeus japonicus
r
2.7.3.3
ATP + L-Arg
-
642448
Portunus trituberculatus
ADP + Nomega-phospho-L-Arg
-
642448
Portunus trituberculatus
r
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
-
Penaeus japonicus
2.7.3.3
?
x * 40000, SDS-PAGE; x * 40500, SDS-PAGE
Portunus trituberculatus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.3
40
-
-
Portunus trituberculatus
2.7.3.3
42
-
-
Penaeus japonicus
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.3.3
10
60
10°C: about 50% of maximal activity, 60°C: about 60% of maximal activity
Penaeus japonicus
2.7.3.3
10
60
10°C: about 40% of maximal activity, 60°C: about 40% of maximal activity
Portunus trituberculatus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.3.3
20
-
10 min, stable up to
Penaeus japonicus
2.7.3.3
20
-
10 min, stable up to
Portunus trituberculatus
2.7.3.3
25
-
10 min, inactivation above
Penaeus japonicus
2.7.3.3
25
-
10 min, inactivation above
Portunus trituberculatus
2.7.3.3
40
-
10 min, 100 mM Tris-HCl buffer, pH 8.0, 15% loss of activity. 10 min, 100 mM Tris-HCl buffer, pH 7.0, 16% loss of activity
Penaeus japonicus
2.7.3.3
40
-
10 min, 100 mM Tris-HCl buffer, pH 8.0, 34% loss of activity. 10 min, 100 mM Tris-HCl buffer, pH 7.0, 46% loss of activity
Portunus trituberculatus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
5.8
-
synthesis of ATP
Portunus trituberculatus
2.7.3.3
6.1
-
synthesis of ATP
Penaeus japonicus
2.7.3.3
9
-
synthesis of N-phospho-L-arginine
Penaeus japonicus
2.7.3.3
9.2
-
synthesis of N-phospho-L-arginine
Portunus trituberculatus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.3
5.5
8
pH 5.5: about 90% of maximal activity, pH 8.0: about 70% of maximal activity, no activity at pH 5.0, synthesis of ATP
Penaeus japonicus
2.7.3.3
5.5
8
pH 5.5: about 90% of maximal activity, pH 8.0: about 70% of maximal activity, no activity at pH 5.0, synthesis of ATP
Portunus trituberculatus
2.7.3.3
8
10.5
pH 8.0: about 60% of maximal activity, pH 10.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg
Penaeus japonicus
2.7.3.3
8
10.5
pH 8.0: about 60% of maximal activity, pH 10.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg
Portunus trituberculatus
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.3.3
more stable in 100 mM Tris-HCl buffer at pH 8.0 than in 100 mM phosphate buffer at pH 7.0
Penaeus japonicus
2.7.3.3
more stable in 100 mM Tris-HCl buffer at pH 8.0 than in 100 mM phosphate buffer at pH 7.0
Portunus trituberculatus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.3
Ca2+
4 mM, activation
Penaeus japonicus
2.7.3.3
Ca2+
4 mM, activation
Portunus trituberculatus
2.7.3.3
Co2+
4 mM, activation
Penaeus japonicus
2.7.3.3
Co2+
4 mM, activation
Portunus trituberculatus
2.7.3.3
Fe2+
4 mM, activation
Penaeus japonicus
2.7.3.3
Fe2+
4 mM, activation
Portunus trituberculatus
2.7.3.3
Mg2+
4 mM, activation; highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP
Penaeus japonicus
2.7.3.3
Mg2+
4 mM, activation; highest activity if ratio Mg2+:ATP is 1:1, synthesis of arginine phosphate; highest activity if the ratio Mg2+:ADP is 4:1, synthesis of ATP
Portunus trituberculatus
2.7.3.3
Mn2+
4 mM, activation; less effective than Mg2+ in activation
Penaeus japonicus
2.7.3.3
Mn2+
4 mM, activation; less effective than Mg2+ in activation
Portunus trituberculatus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.3
40000
-
x * 40000, SDS-PAGE
Portunus trituberculatus
2.7.3.3
40500
-
x * 40500, SDS-PAGE
Portunus trituberculatus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.3
-
Penaeus japonicus
2.7.3.3
-
Portunus trituberculatus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.3.3
234
-
-
Portunus trituberculatus
2.7.3.3
318
-
-
Penaeus japonicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.3.3
-20°C, 10 mM Tris-HCl buffer, pH 8.5, activity gradually decreases, but can be restored by addition of 1 mM 2-mercaptoethanol
Penaeus japonicus
2.7.3.3
-20°C, 10 mM Tris-HCl buffer, pH 8.5, activity gradually decreases, but can be restored by addition of 1 mM 2-mercaptoethanol
Portunus trituberculatus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.3
ATP + L-Arg
-
642448
Penaeus japonicus
ADP + Nomega-phospho-L-Arg
-
642448
Penaeus japonicus
r
2.7.3.3
ATP + L-Arg
-
642448
Portunus trituberculatus
ADP + Nomega-phospho-L-Arg
-
642448
Portunus trituberculatus
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.3
?
-
Penaeus japonicus
2.7.3.3
?
x * 40000, SDS-PAGE; x * 40500, SDS-PAGE
Portunus trituberculatus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.3
40
-
-
Portunus trituberculatus
2.7.3.3
42
-
-
Penaeus japonicus
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
2.7.3.3
10
60
10°C: about 50% of maximal activity, 60°C: about 60% of maximal activity
Penaeus japonicus
2.7.3.3
10
60
10°C: about 40% of maximal activity, 60°C: about 40% of maximal activity
Portunus trituberculatus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.3.3
20
-
10 min, stable up to
Penaeus japonicus
2.7.3.3
20
-
10 min, stable up to
Portunus trituberculatus
2.7.3.3
25
-
10 min, inactivation above
Penaeus japonicus
2.7.3.3
25
-
10 min, inactivation above
Portunus trituberculatus
2.7.3.3
40
-
10 min, 100 mM Tris-HCl buffer, pH 8.0, 15% loss of activity. 10 min, 100 mM Tris-HCl buffer, pH 7.0, 16% loss of activity
Penaeus japonicus
2.7.3.3
40
-
10 min, 100 mM Tris-HCl buffer, pH 8.0, 34% loss of activity. 10 min, 100 mM Tris-HCl buffer, pH 7.0, 46% loss of activity
Portunus trituberculatus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.3
5.8
-
synthesis of ATP
Portunus trituberculatus
2.7.3.3
6.1
-
synthesis of ATP
Penaeus japonicus
2.7.3.3
9
-
synthesis of N-phospho-L-arginine
Penaeus japonicus
2.7.3.3
9.2
-
synthesis of N-phospho-L-arginine
Portunus trituberculatus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.3
5.5
8
pH 5.5: about 90% of maximal activity, pH 8.0: about 70% of maximal activity, no activity at pH 5.0, synthesis of ATP
Penaeus japonicus
2.7.3.3
5.5
8
pH 5.5: about 90% of maximal activity, pH 8.0: about 70% of maximal activity, no activity at pH 5.0, synthesis of ATP
Portunus trituberculatus
2.7.3.3
8
10.5
pH 8.0: about 60% of maximal activity, pH 10.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg
Penaeus japonicus
2.7.3.3
8
10.5
pH 8.0: about 60% of maximal activity, pH 10.0: about 70% of maximal activity, synthesis of N-phospho-L-Arg
Portunus trituberculatus