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Literature summary extracted from

  • Hall, N.; Addis, P.; DeLuca, M.
    Mitochondrial creatine kinase. Physical and kinetic properties of the purified enzyme from beef heart (1979), Biochemistry, 18, 1745-1751.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.015
-
 ADP 30°C Bos taurus
2.7.3.2 0.015
-
 ADP ADP in form of MgADP- Bos taurus
2.7.3.2 0.056
-
 ATP 30°C Bos taurus
2.7.3.2 0.056
-
 ATP ATP in form of MgATP2- Bos taurus
2.7.3.2 0.31
-
 creatine phosphate 30°C Bos taurus
2.7.3.2 4.5
-
 Creatine 30°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.2 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.2 Mg2+ required as MgATP Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.2 35000
-
 2 * 35000, SDS-PAGE Bos taurus
2.7.3.2 64000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.2
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.2 additional information
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.3.2 4°C, 20 mM sodium phosphate buffer, pH 8.0, 10% loss of activity in 2 weeks, faster inactivation in presence of dithiothreitol Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ADP + phosphocreatine
-
 Bos taurus ATP + creatine
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Bos taurus ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine
-
 Bos taurus ADP + phosphocreatine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.2 dimer 2 * 35000, SDS-PAGE Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.2 6 6.5 synthesis of MgATP2- Bos taurus
2.7.3.2 8
-
 synthesis of phosphocreatine Bos taurus