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Literature summary extracted from

  • Blum, H.E.; Deus, B.; Gerok, W.
    Mitochondrial creatine kinase from human heart muscle: purification and characterization of the crystallized isoenzyme (1983), J. Biochem., 94, 1247-1257.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.3.2 isozyme MiMi-CK Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.3.2 0.15
-
 ADP ADP in form of MgADP- Homo sapiens
2.7.3.2 1.7
-
 ATP ATP in form of MgATP2- Homo sapiens
2.7.3.2 3
-
 creatine phosphate
-
 Homo sapiens
2.7.3.2 8
-
 Creatine
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.2 mitochondrion
-
 Gallus gallus 5739
-
2.7.3.2 mitochondrion
-
 Homo sapiens 5739
-
2.7.3.2 mitochondrion
-
 Rattus norvegicus 5739
-
2.7.3.2 mitochondrion
-
 Sus scrofa 5739
-
2.7.3.2 mitochondrion
-
 Bos taurus 5739
-
2.7.3.2 mitochondrion
-
 Oryctolagus cuniculus 5739
-
2.7.3.2 mitochondrion
-
 Papio anubis 5739
-
2.7.3.2 mitochondrion
-
 trout 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.3.2 Mg2+ required as MgATP Gallus gallus
2.7.3.2 Mg2+ required as MgATP Homo sapiens
2.7.3.2 Mg2+ required as MgATP Rattus norvegicus
2.7.3.2 Mg2+ required as MgATP Sus scrofa
2.7.3.2 Mg2+ required as MgATP Bos taurus
2.7.3.2 Mg2+ required as MgATP Oryctolagus cuniculus
2.7.3.2 Mg2+ required as MgATP Papio anubis
2.7.3.2 Mg2+ required as MgATP trout

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.2 42000
-
 2 * 42000, SDS-PAGE Homo sapiens
2.7.3.2 84000 85000 isozyme MiMi-CK, sedimentation equilibrium centrifugation, gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Bos taurus
-
-
-
2.7.3.2 Gallus gallus
-
-
-
2.7.3.2 Homo sapiens
-
-
-
2.7.3.2 Oryctolagus cuniculus
-
-
-
2.7.3.2 Papio anubis
-
 monkey
-
2.7.3.2 Rattus norvegicus
-
-
-
2.7.3.2 Sus scrofa
-
-
-
2.7.3.2 trout
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.2
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 heart
-
 Gallus gallus
-
2.7.3.2 heart
-
 Homo sapiens
-
2.7.3.2 heart
-
 Rattus norvegicus
-
2.7.3.2 heart
-
 Sus scrofa
-
2.7.3.2 heart
-
 Bos taurus
-
2.7.3.2 heart
-
 Oryctolagus cuniculus
-
2.7.3.2 heart
-
 Papio anubis
-
2.7.3.2 heart
-
 trout
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.2 15
-
 synthesis of phosphocreatine Homo sapiens
2.7.3.2 49.5
-
 synthesis of ATP Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.3.2 4°C, pH 7.0, 1 mM dithiothreitol or 14 mM 2-mercaptoethanol, at least 3 months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ADP + phosphocreatine
-
 Homo sapiens ATP + creatine
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Gallus gallus ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Rattus norvegicus ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Sus scrofa ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Bos taurus ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Oryctolagus cuniculus ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Papio anubis ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- trout ADP + creatine phosphate
-
 r
2.7.3.2 ATP + creatine ATP required as MgATP2- Homo sapiens ADP + creatine phosphate in the reverse direction ADP can be replaced by IDP with 18% efficiency, ADP cannot be replaced by GDP, CDP, UDP, dTDP r
2.7.3.2 ATP + creatine
-
 Homo sapiens ADP + phosphocreatine
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.3.2 dimer
-
 Gallus gallus
2.7.3.2 dimer
-
 Rattus norvegicus
2.7.3.2 dimer
-
 Sus scrofa
2.7.3.2 dimer
-
 Bos taurus
2.7.3.2 dimer
-
 Oryctolagus cuniculus
2.7.3.2 dimer
-
 Papio anubis
2.7.3.2 dimer
-
 trout
2.7.3.2 dimer 2 * 42000, SDS-PAGE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.3.2 6.7
-
 synthesis of MgATP2- Homo sapiens
2.7.3.2 8.7
-
 synthesis of phosphocreatine Gallus gallus
2.7.3.2 8.7
-
 synthesis of phosphocreatine Homo sapiens
2.7.3.2 8.7
-
 synthesis of phosphocreatine Rattus norvegicus
2.7.3.2 8.7
-
 synthesis of phosphocreatine Sus scrofa
2.7.3.2 8.7
-
 synthesis of phosphocreatine Bos taurus
2.7.3.2 8.7
-
 synthesis of phosphocreatine Oryctolagus cuniculus
2.7.3.2 8.7
-
 synthesis of phosphocreatine Papio anubis
2.7.3.2 8.7
-
 synthesis of phosphocreatine trout

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.3.2 4.5 10.5 rapid inactivation above and below Homo sapiens