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Literature summary extracted from

  • Brooks, S.P.; Bennett, V.D.; Suelter, C.H.
    Homogeneous chicken heart mitochondrial creatine kinase purified by dye-ligand and transition-state analog-affinity chromatography (1987), Anal. Biochem., 164, 190-198.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.3.2 mitochondrion
-
Gallus gallus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.3.2 43000
-
2 * 43000, SDS-PAGE Gallus gallus
2.7.3.2 84000
-
isozyme MiMi-CK, equilibrium centrifugation Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.7.3.2 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.3.2
-
Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.3.2 heart
-
Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.3.2 250
-
30°C, pH 7.0 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.7.3.2 4°C, 10 mM MOPS buffer, pH 7.2, 2% v/v glycerol, 25 mM 2-mercaptoethanol, 0.1 mM EDTA, stable for more than 4 months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.3.2 ATP + creatine
-
Gallus gallus ADP + creatine phosphate
-
r

Subunits

EC Number Subunits Comment Organism
2.7.3.2 dimer 2 * 43000, SDS-PAGE Gallus gallus