BRENDA - Enzyme Database

Purification and properties of guanidinoacetate kinase from a polychaete, Perineis sp.

Shirokane, Y.; Nakajima, M.; Mizusawa, K.; Agric. Biol. Chem. 55, 2235-2242 (1991)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.1
3-guanidinopropionate
slight
Perinereis sp.
2.7.3.1
4-Guanidinobutyrate
slight
Perinereis sp.
2.7.3.1
5,5'-dithiobis(2-nitrobenzoate)
-
Perinereis sp.
2.7.3.1
agmatine
-
Perinereis sp.
2.7.3.1
AMP
-
Perinereis sp.
2.7.3.1
Ca2+
-
Perinereis sp.
2.7.3.1
Creatine
slight
Perinereis sp.
2.7.3.1
guanidine
slight
Perinereis sp.
2.7.3.1
Hg2+
-
Perinereis sp.
2.7.3.1
iodoacetamide
-
Perinereis sp.
2.7.3.1
L-arginine
slight
Perinereis sp.
2.7.3.1
methylguanidine
slight
Perinereis sp.
2.7.3.1
N-bromosuccinimide
-
Perinereis sp.
2.7.3.1
p-chloromercuribenzoate
-
Perinereis sp.
2.7.3.1
Tauroguanine
slight
Perinereis sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.1
0.8
-
ATP
pH 8.1, 25°C
Perinereis sp.
2.7.3.1
4.1
-
Guanidinoacetate
pH 8.1, 25°C
Perinereis sp.
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.1
Ca2+
29% of Mg2+-activation
Perinereis sp.
2.7.3.1
Mg2+
5 mM, activation
Perinereis sp.
2.7.3.1
Mn2+
5 mM, activation
Perinereis sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.1
45000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
2.7.3.1
47000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
2.7.3.1
90000
-
gel filtration
Perinereis sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.1
ATP + guanidinoacetate
Perinereis sp.
-
ADP + phosphoguanidinoacetate
-
Perinereis sp.
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.3.1
Perinereis sp.
-
polychaete
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.3.1
-
Perinereis sp.
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.3.1
45.3
-
pH 8.1, 25°C
Perinereis sp.
Storage Stability
EC Number
Storage Stability
Organism
2.7.3.1
-20°C, 20 mM Tris-acetate buffer, pH 8.0, 1 mM dithiothreitol, 5% glycerol, several months
Perinereis sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.1
ATP + guanidinoacetate
-
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
2.7.3.1
ATP + guanidinoacetate
specific for guanidinoacetate, ITP 2%, GTP 0.5% of ATP activity
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
Subunits
EC Number
Subunits
Commentary
Organism
2.7.3.1
dimer
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.1
35
-
phosphorylation of guanidinoacetate
Perinereis sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.3.1
35
-
stable up to
Perinereis sp.
2.7.3.1
40
-
20 min, 4% loss of activity
Perinereis sp.
2.7.3.1
45
-
20 min, 28% loss of activity
Perinereis sp.
2.7.3.1
50
-
inactivation
Perinereis sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.1
8.1
-
phosphorylation of guanidinoacetate
Perinereis sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.1
6.5
9
less than 50% of maximal activity above and below
Perinereis sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.3.1
5.5
9.5
-
Perinereis sp.
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.3.1
3-guanidinopropionate
slight
Perinereis sp.
2.7.3.1
4-Guanidinobutyrate
slight
Perinereis sp.
2.7.3.1
5,5'-dithiobis(2-nitrobenzoate)
-
Perinereis sp.
2.7.3.1
agmatine
-
Perinereis sp.
2.7.3.1
AMP
-
Perinereis sp.
2.7.3.1
Ca2+
-
Perinereis sp.
2.7.3.1
Creatine
slight
Perinereis sp.
2.7.3.1
guanidine
slight
Perinereis sp.
2.7.3.1
Hg2+
-
Perinereis sp.
2.7.3.1
iodoacetamide
-
Perinereis sp.
2.7.3.1
L-arginine
slight
Perinereis sp.
2.7.3.1
methylguanidine
slight
Perinereis sp.
2.7.3.1
N-bromosuccinimide
-
Perinereis sp.
2.7.3.1
p-chloromercuribenzoate
-
Perinereis sp.
2.7.3.1
Tauroguanine
slight
Perinereis sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.3.1
0.8
-
ATP
pH 8.1, 25°C
Perinereis sp.
2.7.3.1
4.1
-
Guanidinoacetate
pH 8.1, 25°C
Perinereis sp.
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.3.1
Ca2+
29% of Mg2+-activation
Perinereis sp.
2.7.3.1
Mg2+
5 mM, activation
Perinereis sp.
2.7.3.1
Mn2+
5 mM, activation
Perinereis sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.3.1
45000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
2.7.3.1
47000
-
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
2.7.3.1
90000
-
gel filtration
Perinereis sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.3.1
ATP + guanidinoacetate
Perinereis sp.
-
ADP + phosphoguanidinoacetate
-
Perinereis sp.
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.3.1
-
Perinereis sp.
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.3.1
45.3
-
pH 8.1, 25°C
Perinereis sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.3.1
-20°C, 20 mM Tris-acetate buffer, pH 8.0, 1 mM dithiothreitol, 5% glycerol, several months
Perinereis sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.3.1
ATP + guanidinoacetate
-
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
2.7.3.1
ATP + guanidinoacetate
specific for guanidinoacetate, ITP 2%, GTP 0.5% of ATP activity
642364
Perinereis sp.
ADP + phosphoguanidinoacetate
-
642364
Perinereis sp.
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.3.1
dimer
1 * 47000 + 1 * 45000, SDS-PAGE
Perinereis sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.7.3.1
35
-
phosphorylation of guanidinoacetate
Perinereis sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.7.3.1
35
-
stable up to
Perinereis sp.
2.7.3.1
40
-
20 min, 4% loss of activity
Perinereis sp.
2.7.3.1
45
-
20 min, 28% loss of activity
Perinereis sp.
2.7.3.1
50
-
inactivation
Perinereis sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.3.1
8.1
-
phosphorylation of guanidinoacetate
Perinereis sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.3.1
6.5
9
less than 50% of maximal activity above and below
Perinereis sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.3.1
5.5
9.5
-
Perinereis sp.