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Literature summary extracted from

  • Farago, A.; Denes, G.
    Mechanism of arginine biosynthesis in Chlamydomonas reinhardti. II. Purification and properties of N-acetylglutamate 5-phosphotransferase, the allosteric enzyme of the pathway (1967), Biochim. Biophys. Acta, 136, 6-18.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.2.8 L-arginine protects against inactivating effects of high concentrations of urea Chlamydomonas reinhardtii
2.7.2.8 L-arginine protects against the inactivating effects of heat Chlamydomonas reinhardtii
2.7.2.8 urea, 4.0 M, complete loss of activity after 120 min Chlamydomonas reinhardtii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.8 arginine pH-optimum for inhibition is 7.5 Chlamydomonas reinhardtii
2.7.2.8 L-canavanine
-
 Chlamydomonas reinhardtii
2.7.2.8 L-citrulline
-
 Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.8 1.6
-
 ATP pH 5.5, 37°C Chlamydomonas reinhardtii
2.7.2.8 15
-
 N-acetyl-L-glutamate pH 5.5, 37°C Chlamydomonas reinhardtii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.8 Co2+ Mg2+ or Co2+ required Chlamydomonas reinhardtii
2.7.2.8 Mg2+ Mg2+ or Co2+ required Chlamydomonas reinhardtii
2.7.2.8 Mn2+ less effective than Mg2+ and Co2+ in activation Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate Chlamydomonas reinhardtii key enzyme in regulation of arginine biosynthesis ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.8
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Chlamydomonas reinhardtii ADP + N-acetyl-L-glutamate 5-phosphate in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate ?
2.7.2.8 ATP + N-acetyl-L-glutamate key enzyme in regulation of arginine biosynthesis Chlamydomonas reinhardtii ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.8 37
-
 assay at Chlamydomonas reinhardtii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.8 64
-
 10 min, 75% loss of activity without stabilizer, about 40% loss of activity with 75 mM N-acetyl-L-glutamate, 10 min, 10% loss of activity with 1 mM L-arginine Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.8 5.5
-
-
 Chlamydomonas reinhardtii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.8 4.6 7 pH 4.6: about 75% of maximum activity, pH 7: about 60% of maximum activity Chlamydomonas reinhardtii