Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Twarog, R.; Wolfe, R.S.
    Role of butyryl phosphate in the energy metabolism of Clostridium tetanomorphum (1963), J. Bacteriol., 86, 112-117.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.7 4.23
-
 ATP pH 7.5, 30°C, reaction with butanoate, activation with 6 mM MgSO4 Clostridium tetanomorphum
2.7.2.7 5.06
-
 ATP pH 7.5, 30°C, reaction with butanoate, activation with 6 mM MnSO4 Clostridium tetanomorphum
2.7.2.7 10.8
-
 Butanoate pH 7.5, 30°C, activation with 10 mM MgSO4 Clostridium tetanomorphum
2.7.2.7 14.3
-
 Butanoate pH 7.5, 30°C, activation with 6 mM MnSO4 Clostridium tetanomorphum
2.7.2.7 15.3
-
 valerate pH 7.5, 30°C, activation with 6 mM MnSO4 Clostridium tetanomorphum
2.7.2.7 15.5
-
 valerate pH 7.5, 30°C, activation with 10 mM MgSO4 Clostridium tetanomorphum
2.7.2.7 77
-
 Isobutanoate pH 7.5, 30°C, activation with 10 mM MgSO4 Clostridium tetanomorphum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.7 Mg2+
-
 Clostridium butyricum
2.7.2.7 Mg2+ divalent cation requirement is satisfied by Mg2+ Clostridium tetanomorphum

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.7 Clostridium butyricum
-
-
-
2.7.2.7 Clostridium pasteurianum
-
-
-
2.7.2.7 Clostridium tetanomorphum
-
-
-
2.7.2.7 Clostridium tyrobutyricum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.7 partial Clostridium tetanomorphum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.7 64.3
-
-
 Clostridium tetanomorphum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.7 ATP + butanoate
-
 Clostridium butyricum ADP + butanoyl phosphate
-
 ?
2.7.2.7 ATP + butanoate
-
 Clostridium tetanomorphum ADP + butanoyl phosphate
-
 ?
2.7.2.7 ATP + isobutanoate
-
 Clostridium butyricum ADP + isobutanoyl phosphate
-
 ?
2.7.2.7 ATP + isobutanoate
-
 Clostridium tetanomorphum ADP + isobutanoyl phosphate
-
 ?
2.7.2.7 ATP + propionate
-
 Clostridium butyricum ADP + propanoyl phosphate
-
 ?
2.7.2.7 ATP + propionate
-
 Clostridium tetanomorphum ADP + propanoyl phosphate
-
 ?
2.7.2.7 ATP + valerate
-
 Clostridium butyricum ADP + pentanoyl phosphate
-
 ?
2.7.2.7 ATP + valerate
-
 Clostridium tetanomorphum ADP + pentanoyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.7 7.4 8.3 activation by Mg2+ Clostridium tetanomorphum
2.7.2.7 7.5 7.6 activation by Mn2+ Clostridium tetanomorphum