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Literature summary extracted from

  • Arevalo-Rodriguez, M.; Calderon, I.L.; Holmberg, S.
    Mutations that cause threonine sensitivity identify catalytic and regulatory regions of the aspartate kinase of Saccharomyces cerevisiae (1999), Yeast, 15, 1331-1345.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.4 single aspartate kinase encoded by the HOM3 gene, Escherichia coli DH5alpha as host Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.4 A406T site-directed mutagenesis, 30fold more strongly inhibited by threonine Saccharomyces cerevisiae
2.7.2.4 G25D site-directed mutagenesis, reduced affinity for its substrates aspartate and ATP Saccharomyces cerevisiae
2.7.2.4 K26I site-directed mutagenesis, reduced affinity for its substrates aspartate and ATP Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 L-threonine
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 5.4
-
 ATP mutant hom3-S45 Saccharomyces cerevisiae
2.7.2.4 10.4
-
 ATP mutant hom3-S49 Saccharomyces cerevisiae
2.7.2.4 11.3
-
 L-aspartate mutant hom3-S45 Saccharomyces cerevisiae
2.7.2.4 12.4
-
 L-aspartate mutant hom3-S49 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Saccharomyces cerevisiae first step in the branched pathway leading to synthesis of threonine and methionine from aspartate ADP + 4-phospho-L-aspartate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Saccharomyces cerevisiae
-
 yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 partial Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Saccharomyces cerevisiae ADP + 4-phospho-L-aspartate
-
 r
2.7.2.4 ATP + L-aspartate first step in the branched pathway leading to synthesis of threonine and methionine from aspartate Saccharomyces cerevisiae ADP + 4-phospho-L-aspartate
-
 r