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Literature summary extracted from

  • Wong, K.F.; Dennis, D.T.
    Aspartokinase from wheat germ (1973), Plant Physiol., 51, 322-326.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.2.4 L-lysine and L-threonine partially prevents from heat inactivation Triticum aestivum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.4 DL-2,6-Diaminoheptanoate
-
 Triticum aestivum
2.7.2.4 DL-meso-diaminopimelic acid
-
 Triticum aestivum
2.7.2.4 L-lysine
-
 Triticum aestivum
2.7.2.4 L-threonine concerted inhibition together with L-lysine Triticum aestivum
2.7.2.4 additional information no inhibition with L-homoserine, L-methionine and L-isoleucine Triticum aestivum
2.7.2.4 N-epsilon-formyl-L-lysine
-
 Triticum aestivum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.4 additional information
-
 additional information Km for MgCl2 3.3 mM Triticum aestivum
2.7.2.4 1.2
-
 ATP pH 7.8, 27°C Triticum aestivum
2.7.2.4 1.3
-
 ATP pH 7.8, 27°C Triticum aestivum
2.7.2.4 16.7
-
 L-aspartate pH 7.8, 27°C Triticum aestivum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.4 Mg2+
-
 Triticum aestivum
2.7.2.4 Mn2+
-
 Triticum aestivum
2.7.2.4 additional information Ca2+ shows a trace of activity, Zn2+ is inactive Triticum aestivum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.4 ATP + L-aspartate Triticum aestivum
-
 ADP + 4-phospho-L-aspartate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.4 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.4 partially Triticum aestivum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.4 germ
-
 Triticum aestivum
-

Storage Stability

EC Number Storage Stability Organism
2.7.2.4 -15°C, dissolved in 0.05 M TES buffer containing 30% glycerol, pH 8.0, activity remains unchanged after 2.5 months Triticum aestivum
2.7.2.4 4°C, dissolved in 0.05 M TES buffer containing 30% glycerol, pH 8.0, activity remains unchanged after 6 days Triticum aestivum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.4 ATP + L-aspartate
-
 Triticum aestivum ADP + 4-phospho-L-aspartate
-
 r
2.7.2.4 GTP + L-aspartate
-
 Triticum aestivum GDP + 4-phospho-L-aspartate
-
 r
2.7.2.4 additional information no reaction observed with ITP, CTP or UTP Triticum aestivum ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.4 45
-
 loses 70% of its original activity after heating for 15 min Triticum aestivum