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Literature summary extracted from

  • Bentahir, M.; Feller, G.; Aittaleb, M.; Lamotte-Brasseur, J.; Himri, T.; Chessa, J.P.; Gerday, C.
    Structural, kinetic, and calorimetric characterization of the cold-active phosphoglycerate kinase from the antarctic Pseudomonas sp. TACII18 (2000), J. Biol. Chem., 275, 11147-11153.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.3 gene pgk, DNA and amino acid sequence determination, overexpression as native and His-tagged wild-type enzyme in Escherichia coli BL21(DE3), the His-tag strongly alters the enzymes properties, e.g. thermostability, kinetic parameters like kcat and Km Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 SO42- above 50 mM Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.21
-
 ATP recombinant wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
2.7.2.3 0.24
-
 ATP native wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
2.7.2.3 0.38
-
 ATP recombinant His-tagged enzyme, pH 7.5, 25°C Pseudomonas sp.
2.7.2.3 0.48
-
 3-phospho-D-glycerate native wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
2.7.2.3 0.53
-
 3-phospho-D-glycerate recombinant wild-type enzyme, pH 7.5, 25°C Pseudomonas sp.
2.7.2.3 0.89
-
 3-phospho-D-glycerate recombinant His-tagged enzyme, pH 7.5, 25°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 40000
-
 x * 40000, recombinant wild-type, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Pseudomonas sp. Q9RBS3 gene pgk, strain TACII18
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 native wild-type from Pseudomonas sp., and recombinant native and His-tagged enzyme from Escherichia coli as , to homogeneity Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 550
-
 purified recombinant His-tagged enzyme Pseudomonas sp.
2.7.2.3 750
-
 purified recombinant wild-type and native wild-type enzyme Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Pseudomonas sp. ADP + 1,3-diphosphoglycerate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 ? x * 40000, recombinant wild-type, SDS-PAGE Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 additional information
-
 the recombinant His-tagged wild-type enzyme shows reduced thermostability compared to the native and recombinant wild-type without tag Pseudomonas sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information temperature-dependence of kinetic Pseudomonas sp.
2.7.2.3 362
-
 3-phospho-D-glycerate recombinant His-tagged enzyme Pseudomonas sp.
2.7.2.3 411
-
 ATP recombinant His-tagged enzyme Pseudomonas sp.
2.7.2.3 500
-
 ATP recombinant wild-type enzyme Pseudomonas sp.
2.7.2.3 519
-
 ATP native wild-type enzyme Pseudomonas sp.
2.7.2.3 537
-
 3-phospho-D-glycerate recombinant wild-type enzyme Pseudomonas sp.
2.7.2.3 540
-
 3-phospho-D-glycerate native wild-type enzyme Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7.5
-
 assay at Pseudomonas sp.
2.7.2.3 8
-
 recombinant wild-type Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Pseudomonas sp.
2.7.2.3 ATP required as phosphate donor Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.3 Pseudomonas sp. recombinant wild-type
-
 4.9