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Literature summary extracted from

  • Bernstein, B.E.; Hol, W.G.
    Crystal structures of substrates and products bound to the phosphoglycerate kinase active site reveal the catalytic mechanism (1998), Biochemistry, 37, 4429-4436.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 purified, recombinant enzyme is crystallized as a ternary complex by vapour diffusion method, 10°C, protein solution: 6 mg/ml, 10 mM 3-phospho-D-glycerate, 10 mM MgADP, 25 mM Tris, pH 7.5, 50 mM NaCl, 10 m DTT, plus equal volume of 2.5 M sodium potassium phosphate, pH 8.0 Trypanosoma brucei
2.7.2.3 purified, recombinant enzyme is crystallized in complex with nucleotide analogue Mg-beta,gamma-imido-adenosine-5'-triphosphate by vapour diffusion method, 10°C, protein solution: 6 mg/ml, 10 mM 3-phospho-D-glycerate, 10 mM MgADP-, 25 mM Tris, pH 7.5, 50 mM NaCl, 10 m DTT, plus equal volume of 2.5 M sodium potassium phosphate, pH 7.5 Trypanosoma brucei
2.7.2.3 structure determination and analysis Trypanosoma brucei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 180000
-
ternary complex of 2 dimers build of 4 enzyme molecules, crystal structure determination Trypanosoma brucei

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Trypanosoma brucei
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.2.3 ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate mechanism Trypanosoma brucei
2.7.2.3 ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate substrate binding Trypanosoma brucei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
Trypanosoma brucei ADP + 1,3-diphosphoglycerate
-
r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 More ternary structure Trypanosoma brucei
2.7.2.3 More structure model Trypanosoma brucei

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
Trypanosoma brucei
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Trypanosoma brucei
2.7.2.3 ATP required as phosphate donor Trypanosoma brucei