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Literature summary extracted from

  • Yoshida, A.; Watanabe, S.
    Human phosphoglycerate kinase. I. Crystallization and characterization of normal enzyme (1972), J. Biol. Chem., 247, 440-445.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 crystallization via precipitation with ammonium sulfate stepwise from 30-35% to 65% w/v within 24 h Homo sapiens

General Stability

EC Number General Stability Organism
2.7.2.3 dissolved cyrstallized enzyme at low concentration is rapidly inactivated at neutral pH Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 phosphate phosphate buffer Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.3 divalent cation Mg2+ or Mn2+ Homo sapiens
2.7.2.3 Mg2+ 5 mM Mg2+ or 10 mM Mn2+ required Homo sapiens
2.7.2.3 Mn2+ Mn2+ or Mg2+ required Homo sapiens
2.7.2.3 Mn2+ 5 mM Mg2+ or 10 mM Mn2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 49600
-
 sedimentation equilibrium method Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 650 700 purified enzyme, at 25°C Homo sapiens
2.7.2.3 1000
-
 purified enzyme, at 35°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.7.2.3 4°C, with 10 mg/ml bovine serum albumin, stable for at least 1 week Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Homo sapiens ADP + 1,3-diphosphoglycerate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 25
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7.2 9
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Homo sapiens
2.7.2.3 ATP required as phosphate donor Homo sapiens