Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Pegorado, B.; Lee, C.Y.
    Purification and characterization of two isozymes of 3-phosphoglycerate kinase from the mouse (1978), Biochim. Biophys. Acta, 522, 423-433.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.3 iodoacetate
-
 Mus musculus
2.7.2.3 N-ethylmaleimide
-
 Mus musculus
2.7.2.3 Urea
-
 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 additional information
-
 additional information pH-dependence of Km values for the substrates in forward and reverse reaction, 2 isozymes Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 45000
-
 gel filtration Mus musculus
2.7.2.3 45000
-
 isozymes PGKA and PGKB Mus musculus
2.7.2.3 47000
-
 1 * 47000, isozymes PGKA and PGKB, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Mus musculus
-
 2 isozymes PGKA and PGKB
-
2.7.2.3 Mus musculus
-
 DBA/2J
-
2.7.2.3 Mus musculus DBA/2J
-
 DBA/2J
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 isozymes PGKA and PGKB Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 muscle isozyme PGKA Mus musculus
-
2.7.2.3 testis isozyme PGKB Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 260 450 purified isozyme PGKB Mus musculus
2.7.2.3 500
-
 purified isozyme PGKA Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Mus musculus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Mus musculus DBA/2J ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 CTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 CTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus DBA/2J CDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dATP + 3-phospho-D-glycerate
-
 Mus musculus dADP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 dATP + 3-phospho-D-glycerate
-
 Mus musculus DBA/2J dADP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate 60% of the activity with ATP Mus musculus GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 GTP + 3-phospho-D-glycerate 60% of the activity with ATP Mus musculus DBA/2J GDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus UDP + 1,3-diphosphoglycerate
-
 ?
2.7.2.3 UTP + 3-phospho-D-glycerate 1% of the activity with ATP Mus musculus DBA/2J UDP + 1,3-diphosphoglycerate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.3 monomer 1 * 47000, isozymes PGKA and PGKB, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.3 40
-
-
 Mus musculus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.2.3 25 40 activity of both isozymes in either reaction direction increases steadily with increasing temperature within this range Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 50
-
 pH 6.0, 10 min: 20% loss of activity, PGKA, 80% loss of activity PGKB Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 6.5
-
-
 Mus musculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.3 4.7
-
 isozyme PGKB: loss of 70% activity after 45 min, isozyme PGKA: loss of 10% activity after 45 min Mus musculus
2.7.2.3 9.6
-
 isozymes PGKA and PGKB: stable for at least 30 min Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Mus musculus
2.7.2.3 ATP required as phosphate donor Mus musculus
2.7.2.3 CTP
-
 Mus musculus
2.7.2.3 dATP
-
 Mus musculus
2.7.2.3 GTP
-
 Mus musculus
2.7.2.3 UTP
-
 Mus musculus