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Literature summary extracted from

  • Stewart, A.A.; Scopes, R.K.
    Phosphoglycerate kinase B from ram testis. Purification, characterisation and comparison with the muscle isoenzyme (1978), Eur. J. Biochem., 85, 89-95.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.3 0.09
-
 ATP pH 7.5, isozyme PGKA, in presence of 0.05 mM 1,3-bisphosphoglycerate Ovis aries
2.7.2.3 0.09
-
 ATP ATP in form of MgATP2- Ovis aries
2.7.2.3 0.095
-
 ATP pH 7.5, isozyme PGKB, in presence of 0.05 mM 1,3-bisphosphoglycerate Ovis aries
2.7.2.3 0.095
-
 ATP ATP in form of MgATP2- Ovis aries

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.3 47000
-
 isozyme PGKA and PGKB, tryptophan content Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Ovis aries
-
 ram
-
2.7.2.3 Ovis aries
-
 testis-specific isozyme PGKB, isozyme PGKA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3 2 isozymes: PGKA and PGKB, from testis to homogeneity Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.2.3 muscle isozyme PGKA Ovis aries
-
2.7.2.3 testis testis-specific isozyme PGKB, isozyme PGKA Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.3 700
-
 purified isozyme PGKA Ovis aries
2.7.2.3 720
-
 purified isozyme PGKB Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Ovis aries ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Ovis aries ADP + 3-phospho-D-glyceroyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.3 ? x * 47000, SDS-PAGE Ovis aries
2.7.2.3 monomer 1 * 47000-47500, isozymes PGKA and PGKB, SDS-PAGE Ovis aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.3 additional information
-
 thermal denaturation at pH 5.5 and pH 7.5 of the isoenzymes Ovis aries

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.3 7.5
-
 assay at Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Ovis aries
2.7.2.3 ATP true substrate is the magnesium complexes of ATP Ovis aries
2.7.2.3 ATP required as phosphate donor Ovis aries