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Literature summary extracted from

  • Davies, G.J.; Gamblin, S.J.; Littlechild, J.A.; Watson, H.C.
    Purification, crystallization and preliminary X-ray analysis of the 3-phosphoglycerate kinase from Bacillus stearothermophilus (1992), J. Mol. Biol., 227, 1263-1264.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.3 hanging drop vapour phase diffusion method, 18°C, protein solution: 15 mg/ml, Tris-HCl, pH 7.0, 1 mM MgATP2-, 0.1 mM NaN3, 10% w/v polyethylene glycol 600, precipitant is 40% w/v polyethylene glycol 600, X-ray diffraction structure determination and analysis Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.3 Geobacillus stearothermophilus
-
 NCA 1503
-
2.7.2.3 Geobacillus stearothermophilus NCA 1503
-
 NCA 1503
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.3
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Geobacillus stearothermophilus ADP + 1,3-diphosphoglycerate
-
 r
2.7.2.3 ATP + 3-phospho-D-glycerate
-
 Geobacillus stearothermophilus NCA 1503 ADP + 1,3-diphosphoglycerate
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.2.3 ADP
-
 Geobacillus stearothermophilus
2.7.2.3 ATP required as phosphate donor Geobacillus stearothermophilus