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Literature summary extracted from

  • Uriarte, M.; Marina, A.; Ramon-Maiques, S.; Fita, I.; Rubio, V.
    The carbamoyl-phosphate synthetase of Pyrococcus furiosus is enzymologically and structurally a carbamate kinase (1999), J. Biol. Chem., 274, 16295-16303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.2
-
 Pyrococcus furiosus
2.7.2.2 expression in Escherichia coli Pyrococcus furiosus
6.3.4.16 expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.2 crystals of the pyrococcal enzyme grown in the absence or presence of MgATP (Fig. 7) diffracted with a conventional X-ray source to at least 2.6 and 2.0 Å resolution, respectively Pyrococcus furiosus
6.3.4.16 recombinant enzyme Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.2 34000
-
 x * 34000, SDS-PAGE Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate Pyrococcus furiosus carbamate kinase with an anabolic role. The extreme living conditions of the pyrococci may render unnecessary the enzymatic synthesis of carbamate by carbamoyl-phosphate synthetase with the associated expenditure of an extra ATP molecule, as a prelude to making carbamoyl phosphate, explaining the use of carbamate kinase for carbamoyl phosphate synthesis in this organisms ATP + NH3 + CO2
-
 ?
6.3.4.16 ATP + NH3 + CO2 + H2O Pyrococcus furiosus the product carbamoylphosphate is used in the biosynethsis of pyrimidines, arginine, and urea ADP + phosphate + carbamoylphosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Pyrococcus furiosus
-
-
-
2.7.2.2 Pyrococcus furiosus P95474
-
-
6.3.4.16 Escherichia coli
-
-
-
6.3.4.16 Pyrococcus furiosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.2
-
 Pyrococcus furiosus
6.3.4.16 recombinant enzyme Pyrococcus furiosus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.16 2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate the enzyme is enzymologically and structurally a carbamate kinase, stoichiometry Pyrococcus furiosus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.16 16
-
 value at 37°C and 60°C Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate
-
 Pyrococcus furiosus ATP + NH3 + CO2
-
 ?
2.7.2.2 ADP + carbamoyl phosphate carbamate kinase with an anabolic role. The extreme living conditions of the pyrococci may render unnecessary the enzymatic synthesis of carbamate by carbamoyl-phosphate synthetase with the associated expenditure of an extra ATP molecule, as a prelude to making carbamoyl phosphate, explaining the use of carbamate kinase for carbamoyl phosphate synthesis in this organisms Pyrococcus furiosus ATP + NH3 + CO2
-
 ?
2.7.2.2 ATP + NH3 + CO2 carbamoyl phosphate synthetase is a carbamate synthase Pyrococcus furiosus ADP + carbamoyl phosphate
-
 r
6.3.4.16 ATP + carbamate
-
 Pyrococcus furiosus ADP + carbamoyl phosphate
-
 r
6.3.4.16 ATP + NH3 + CO2 + H2O
-
 Escherichia coli ADP + phosphate + carbamoyl phosphate
-
 ir
6.3.4.16 ATP + NH3 + CO2 + H2O
-
 Pyrococcus furiosus ADP + phosphate + carbamoyl phosphate
-
 ir
6.3.4.16 ATP + NH3 + CO2 + H2O the product carbamoylphosphate is used in the biosynethsis of pyrimidines, arginine, and urea Pyrococcus furiosus ADP + phosphate + carbamoylphosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.2.2 ? x * 34000, SDS-PAGE Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.7.2.2 CK-like CPS
-
 Pyrococcus furiosus
6.3.4.16 carbamate kinase-like carbamoyl-phosphate synthetase
-
 Pyrococcus furiosus
6.3.4.16 CK-like CPS
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.16 60
-
 extreme thermostable Pyrococcus furiosus