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Literature summary extracted from

  • Ramon-Maiques, S.; Marina, A.; Uriarte, M.; Fita, I.; Rubio, V.
    The 1.5 A resolution crystal structure of the carbamate kinase-like carbamoyl phosphate synthetase from the hyperthermophilic Archaeon pyrococcus furiosus, bound to ADP, confirms that this thermostable enzyme is a carbamate kinase, and provides insight into substrate binding and stability in carbamate kinases (2000), J. Mol. Biol., 299, 463-476.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.2
-
 Pyrococcus furiosus
6.3.4.16 1.5 A resolution structure of recombinant enzyme Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.2 0.001
-
 ADP pH 8.0, 37°C Pyrococcus furiosus
2.7.2.2 0.017
-
 ATP pH 8.0, 37°C Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.16 ATP + NH3 + CO2 + H2O Pyrococcus furiosus the product carbamoyl phosphate is used in the biosynthesis of pyrimidines, arginine, and urea ADP + phosphate + carbamoylphosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Pyrococcus furiosus P95474
-
-
6.3.4.16 Pyrococcus furiosus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.2.2 ATP + NH3 + hydrogencarbonate = ADP + carbamoyl phosphate + H2O mechanism Pyrococcus furiosus
a
6.3.4.16 2 ATP + NH3 + hydrogencarbonate = 2 ADP + phosphate + carbamoyl phosphate the enzyme structure is ill-suited for catalysing the characteristic three-step reaction of carbamoyl-phosphate synthetase and supports the view that the carbamate kinase-like carbamoyl-phosphate synthetase is in fact a highly thermostable and very slow carbamate kinase that, in extreme environment of Pyrococcus furiosus, may have the new function of making, rather than using, carbamoyl phosphate Pyrococcus furiosus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate
-
 Pyrococcus furiosus ATP + NH3 + CO2
-
 r
2.7.2.2 ATP + NH3 + CO2 carbamoyl phosphate synthetase is a carbamate synthase Pyrococcus furiosus ADP + carbamoyl phosphate
-
 r
6.3.4.16 ATP + carbamate
-
 Pyrococcus furiosus ADP + carbamoyl phosphate
-
 r
6.3.4.16 ATP + NH3 + CO2 + H2O three step reaction Pyrococcus furiosus ADP + phosphate + carbamoyl phosphate
-
 ir
6.3.4.16 ATP + NH3 + CO2 + H2O the product carbamoyl phosphate is used in the biosynthesis of pyrimidines, arginine, and urea Pyrococcus furiosus ADP + phosphate + carbamoylphosphate
-
 ir

Subunits

EC Number Subunits Comment Organism
2.7.2.2 dimer homodimer, crystalline structure Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
6.3.4.16 carbamate kinase-like carbamoyl-phosphate synthetase
-
 Pyrococcus furiosus
6.3.4.16 CK-like CPS
-
 Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.4.16 additional information
-
 the enzyme is extreme thermostable Pyrococcus furiosus