Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Marina, A.; Uriarte, M.; Barcelona, B.; Fresquet, V.; Cervera, J.; Rubio, V.
    Carbamate kinase from Enterococcus faecalis and Enterococcus faecium--cloning of the genes, studies on the enzyme expressed in Escherichia coli, and sequence similarity with N-acetyl-L-glutamate kinase (1998), Eur. J. Biochem., 253, 280-291.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.2.2
-
 Enterococcus faecium

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.2
-
 Enterococcus faecium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.2 adenosine(5')hexaphospho(5')adenosine
-
 Enterococcus faecium
2.7.2.2 adenosine(5')pentaphospho(5')adenosine
-
 Enterococcus faecium
2.7.2.2 additional information not inhibitory: adenosine(5')triphospho(5')adenosine, phosphate, phosphonoformate Enterococcus faecium
2.7.2.2 Phosphonoacetate competitive to carbamoyl phosphate Enterococcus faecium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.2 1.7
-
 acetate pH 7.4, 37°C Enterococcus faecium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.2 33000
-
 x * 33000, recombinant protein, SDS-PAGE, x * 328000, deduced from gene sequence, 14 residue C-terminal sequence is important for enzyme activity Enterococcus faecium
2.7.2.2 328000
-
 x * 33000, recombinant protein, SDS-PAGE, x * 328000, deduced from gene sequence, 14 residue C-terminal sequence is important for enzyme activity Enterococcus faecium

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.2 Enterococcus faecium P0A2X7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.2 ADP + carbamoyl phosphate
-
 Enterococcus faecium ATP + NH3 + CO2
-
 r
2.7.2.2 ATP + NH3 + acetate
-
 Enterococcus faecium ADP + ?
-
 r
2.7.2.2 ATP + NH3 + CO2
-
 Enterococcus faecium ADP + carbamoyl phosphate
-
 r
2.7.2.2 ATP + NH3 + formate poor substrate Enterococcus faecium ADP + ?
-
 r
2.7.2.2 ATP + NH3 + propionate poor substrate Enterococcus faecium ADP + ?
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.2 ? x * 33000, recombinant protein, SDS-PAGE, x * 328000, deduced from gene sequence, 14 residue C-terminal sequence is important for enzyme activity Enterococcus faecium

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.2 0.15
-
 Phosphonoacetate pH 7.4, 37°C Enterococcus faecium

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.2.2 Enterococcus faecium isoeletric focusing, recombinant protein
-
 4.75