EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.11 | 5-oxo-L-Pro | 12 mM, 10% inhibition | Pseudomonas aeruginosa | |
2.7.2.11 | ADP | - |
Pseudomonas aeruginosa | |
2.7.2.11 | Cd2+ | 0.1 mM, complete inhibition | Pseudomonas aeruginosa | |
2.7.2.11 | DL-3,4-Didehydroproline | 9 mM, 50% inhibition | Pseudomonas aeruginosa | |
2.7.2.11 | Hg2+ | 0.1 mM, complete inhibition | Pseudomonas aeruginosa | |
2.7.2.11 | iodoacetamide | 0.125 mM, 60% inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects | Pseudomonas aeruginosa | |
2.7.2.11 | L-azetidine-2-carboxylic acid | 3 mM, 50% inhibition | Pseudomonas aeruginosa | |
2.7.2.11 | L-methionine-DL-sulphoximine | competitive with L-glutamate | Pseudomonas aeruginosa | |
2.7.2.11 | L-Orn | 12 mM, 10% inhibition | Pseudomonas aeruginosa | |
2.7.2.11 | L-Pro | enzyme from strain PAO1: 5 mM, 50% inhibition, complete inhibition at 30 mM, noncompetitive. Strain PAO 879, a proline-auxotroph mutant lacks a proline-inhibitable gamma-glutamyl kinase | Pseudomonas aeruginosa | |
2.7.2.11 | L-Thioproline | 12 mM, 10% inhibition | Pseudomonas aeruginosa | |
2.7.2.11 | Mg2+ | above 20 mM | Pseudomonas aeruginosa | |
2.7.2.11 | Mn2+ | above 20 mM | Pseudomonas aeruginosa | |
2.7.2.11 | NEM | 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects | Pseudomonas aeruginosa | |
2.7.2.11 | PCMB | 0.125 mM, complete inhibition. Preincubation with 0.25 mM dithiothreitol for 5 min partially protects | Pseudomonas aeruginosa | |
2.7.2.11 | phosphate | - |
Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.11 | 12 | - |
L-glutamate | - |
Pseudomonas aeruginosa |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.11 | Mg2+ | required | Pseudomonas aeruginosa | |
2.7.2.11 | Mn2+ | can partially replace Mg2+ | Pseudomonas aeruginosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.2.11 | 84000 | - |
gel filtration | Pseudomonas aeruginosa |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.11 | Pseudomonas aeruginosa | - |
- |
- |
2.7.2.11 | Pseudomonas aeruginosa PAO 1 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.11 | partial | Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.2.11 | 12.68 | - |
- |
Pseudomonas aeruginosa |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.2.11 | -40°C, 40% loss of activity within 2 months, then remains stable | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.11 | ATP + 5-ethyl-L-glutamate | 5% of the activity with L-glutamate | Pseudomonas aeruginosa | ? | - |
? | |
2.7.2.11 | ATP + 5-ethyl-L-glutamate | 5% of the activity with L-glutamate | Pseudomonas aeruginosa PAO 1 | ? | - |
? | |
2.7.2.11 | ATP + 5-methyl-L-glutamate | 6% of the activity with L-glutamate | Pseudomonas aeruginosa | ? | - |
? | |
2.7.2.11 | ATP + 5-methyl-L-glutamate | 6% of the activity with L-glutamate | Pseudomonas aeruginosa PAO 1 | ? | - |
? | |
2.7.2.11 | ATP + L-glutamate | - |
Pseudomonas aeruginosa | ADP + L-glutamate 5-phosphate | - |
? | |
2.7.2.11 | ATP + L-glutamate | - |
Pseudomonas aeruginosa PAO 1 | ADP + L-glutamate 5-phosphate | - |
? | |
2.7.2.11 | ATP + L-glutamine | 10% of the activity with L-glutamate | Pseudomonas aeruginosa | ? | - |
? | |
2.7.2.11 | ATP + L-glutamine | 10% of the activity with L-glutamate | Pseudomonas aeruginosa PAO 1 | ? | - |
? | |
2.7.2.11 | GTP + L-glutamate | 10% of the activity with ATP | Pseudomonas aeruginosa | GDP + L-glutamate 5-phosphate | - |
? | |
2.7.2.11 | GTP + L-glutamate | 10% of the activity with ATP | Pseudomonas aeruginosa PAO 1 | GDP + L-glutamate 5-phosphate | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.11 | 6 | 6.3 | - |
Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.7.2.11 | 5.5 | 8.5 | pH 5.5: about 80% of maximal activity, pH 8.5: about 40% of maximal activity | Pseudomonas aeruginosa |