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Literature summary extracted from

  • Yu, L.; Ishida, T.; Ozawa, K.; Akutsu, H.; Horiike, K.
    Purification and characterization of homo- and hetero-dimeric acetate kinases from the sulfate-reducing bacterium Desulfovibrio vulgaris (2001), J. Biochem., 129, 411-421.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 acetyl phosphate product inhibition Desulfovibrio vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 1.12
-
 ADP pH 7.5, 25°C Desulfovibrio vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mg2+
-
 Desulfovibrio vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 47800
-
 1 * 49300 + 1 * 47800, isoenzyme AK-II, alphaSalphaL heterodimer, SDS-PAGE Desulfovibrio vulgaris
2.7.2.1 47800
-
 2 * 47800, isoenzyme AK-I, alphaS2 homodimer, SDS-PAGE Desulfovibrio vulgaris
2.7.2.1 49300
-
 1 * 49300 + 1 * 47800, isoenzyme AK-II, alphaSalphaL heterodimer, SDS-PAGE Desulfovibrio vulgaris
2.7.2.1 76400
-
 isoenzyme AK-I, gel filtration Desulfovibrio vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Desulfovibrio vulgaris
-
 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Desulfovibrio vulgaris Miyazaki F.
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Desulfovibrio vulgaris
-
 Miyazaki F.
-
2.7.2.1 Desulfovibrio vulgaris Miyazaki F.
-
 Miyazaki F.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1 2 isoenzymes purified Desulfovibrio vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 19.6
-
 isoenzyme AK-II Desulfovibrio vulgaris
2.7.2.1 28.3
-
 isoenzyme AK-I Desulfovibrio vulgaris

Storage Stability

EC Number Storage Stability Organism
2.7.2.1 -20°C, can be stored in 50 mM Tris-HCl, pH 7.4, 0.3 M NaCl and 2 mM dithiothreitol for several months without loss of activity Desulfovibrio vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Desulfovibrio vulgaris ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Desulfovibrio vulgaris Miyazaki F. ADP + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.1 dimer 1 * 49300 + 1 * 47800, isoenzyme AK-II, alphaSalphaL heterodimer, SDS-PAGE Desulfovibrio vulgaris
2.7.2.1 dimer 2 * 47800, isoenzyme AK-I, alphaS2 homodimer, SDS-PAGE Desulfovibrio vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7 8.5
-
 Desulfovibrio vulgaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.1 5.5 9.5
-
 Desulfovibrio vulgaris