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Literature summary extracted from

  • Lin, W.R.; Peng, Y.; Lew, S.; Lee, C.C.; Hsu, J.J.; Hamel, J.F.; Demain, A.L.
    Purification and characterization of acetate kinase from Clostridium thermocellum (1998), Tetrahedron, 54, 15915-15925.
No PubMed abstract available

Application

EC Number Application Comment Organism
2.7.2.1 industry enzyme involved in the wasteful production of acetate during conversion of cellulose to ethanol Acetivibrio thermocellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 135
-
 acetate pH 7.2-8.0, 50°C Acetivibrio thermocellus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mg2+
-
 Acetivibrio thermocellus
2.7.2.1 Mn2+
-
 Acetivibrio thermocellus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 46000
-
 2 * 46000, homodimer, SDS-PAGE Acetivibrio thermocellus
2.7.2.1 84000
-
 gel filtration Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Acetivibrio thermocellus
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Acetivibrio thermocellus
-
 ATCC 27405
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 490
-
-
 Acetivibrio thermocellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Acetivibrio thermocellus ADP + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.1 dimer 2 * 46000, homodimer, SDS-PAGE Acetivibrio thermocellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.1 50
-
-
 Acetivibrio thermocellus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.2.1 20 80
-
 Acetivibrio thermocellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 60 80 retains full activity, stable up to 60°C, loses 55% of its activity at 70°C, completely inactivated after 2 h at 80°C Acetivibrio thermocellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7.2 8
-
 Acetivibrio thermocellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.1 6 10
-
 Acetivibrio thermocellus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.1 5 10 quite stable between pH 7.0 to 9.0, only 15% loss in activity at pH 10.0, inactivated rapidly outside this range Acetivibrio thermocellus