Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fox, D.K.; Roseman, S.
    Isolation and characterization of homogeneous acetate kinase from Salmonella typhimurium and Escherichia coli (1986), J. Biol. Chem., 261, 13487-13497.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.2.1 destroyed by repeated freezing and thawing Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 destroyed by repeated freezing and thawing Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 Li+
-
 Escherichia coli
2.7.2.1 Li+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 Na+
-
 Escherichia coli
2.7.2.1 Na+
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 propionic acid competitive inhibitor with respect to acetate Escherichia coli
2.7.2.1 propionic acid
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.07
-
 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.07
-
 ATP pH 7.9, 37°C Escherichia coli
2.7.2.1 0.16
-
 acetyl phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.16
-
 acetyl phosphate pH 7.9, 37°C Escherichia coli
2.7.2.1 0.5
-
 ADP pH 7.9, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 0.5
-
 ADP pH 7.9, 37°C Escherichia coli
2.7.2.1 0.78
-
 ITP pH 7.9, 37°C Escherichia coli
2.7.2.1 0.94
-
 ATP pH 7.9, 37°C Escherichia coli
2.7.2.1 1.1
-
 GTP pH 7.9, 37°C Escherichia coli
2.7.2.1 7
-
 acetate pH 7.9, 37°C Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 7
-
 acetate pH 7.9, 37°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 40000
-
 2 * 40000, SDS-PAGE Escherichia coli
2.7.2.1 41200
-
 2 * 41200, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 68000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 70000
-
 gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Escherichia coli
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Escherichia coli
-
 K235, 1100 thi-
-
2.7.2.1 Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT2, wild-type
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 partial Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.7.2.1 -20°C, relatively stable to storage when frozen, remains active for at least 1 year Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Escherichia coli ADP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Escherichia coli GDP + acetyl phosphate
-
 r
2.7.2.1 ITP + acetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium IDP + acetyl phosphate
-
 r
2.7.2.1 ITP + acetate
-
 Escherichia coli IDP + acetyl phosphate
-
 r
2.7.2.1 additional information neither propionic acid nor butyric acid are active as substrates Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.7.2.1 additional information neither propionic acid nor butyric acid are active as substrates Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.1 dimer 2 * 40000, SDS-PAGE Escherichia coli
2.7.2.1 dimer 2 * 41200, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7.5
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.7.2.1 7.5
-
-
 Escherichia coli