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Literature summary extracted from

  • Suzuki, K.; Nakajima, H.; Imahori, K.
    Acetate kinase from Bacillus stearothermophilus (1982), Methods Enzymol., 90, 179-185.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.2.1 alpha-glycerophosphate
-
Geobacillus stearothermophilus
2.7.2.1 D-fructose 1,6-bisphosphate activates formation of ADP Geobacillus stearothermophilus
2.7.2.1 D-Glucose 1,6-bisphosphate
-
Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.1
-
Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Mg2+
-
Geobacillus stearothermophilus
2.7.2.1 Mn2+
-
Geobacillus stearothermophilus
2.7.2.1 additional information Co2+, Ca2+, Cd2+ and Zn2+ can replace Mg2+ or Mn2+ only partially Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 43000
-
4 * 43000, SDS-PAGE Geobacillus stearothermophilus
2.7.2.1 160000 170000 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Geobacillus stearothermophilus function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase ADP + acetyl phosphate
-
r
2.7.2.1 ATP + acetate Geobacillus stearothermophilus NCA 1503 function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase ADP + acetyl phosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Geobacillus stearothermophilus
-
NCA 1503
-
2.7.2.1 Geobacillus stearothermophilus NCA 1503
-
NCA 1503
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 1530
-
-
Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
Geobacillus stearothermophilus ADP + acetyl phosphate
-
r
2.7.2.1 ATP + acetate function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase Geobacillus stearothermophilus ADP + acetyl phosphate
-
r
2.7.2.1 ATP + acetate
-
Geobacillus stearothermophilus NCA 1503 ADP + acetyl phosphate
-
r
2.7.2.1 ATP + acetate function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase Geobacillus stearothermophilus NCA 1503 ADP + acetyl phosphate
-
r
2.7.2.1 ATP + propionate poor acceptor, 5% relative to acetate at 500 mM Geobacillus stearothermophilus ADP + propionyl phosphate
-
r
2.7.2.1 ATP + propionate poor acceptor, 5% relative to acetate at 500 mM Geobacillus stearothermophilus NCA 1503 ADP + propionyl phosphate
-
r
2.7.2.1 GTP + acetate
-
Geobacillus stearothermophilus GDP + acetyl phosphate
-
r
2.7.2.1 GTP + acetate
-
Geobacillus stearothermophilus NCA 1503 GDP + acetyl phosphate
-
r
2.7.2.1 additional information UTP and CTP are active only partially Geobacillus stearothermophilus ?
-
?
2.7.2.1 additional information UTP and CTP are active only partially Geobacillus stearothermophilus NCA 1503 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.2.1 tetramer 4 * 43000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 65
-
quite heat stable Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7.3
-
-
Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.1 7 8 stable, rapid inactivation at pH values lower than 6.0, rather stable at alkaline pH Geobacillus stearothermophilus