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Literature summary extracted from

  • Harwood, C.S.; Canale-Parola, E.
    Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirochete (1982), J. Bacteriol., 152, 246-254.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.7.2.14 complete loss of activity if filtered through columns of Sephacryl S-300 at room temperature Spirochaeta sp.
2.7.2.14 dialysis, 6 h at 5°C, significant loss of activity Spirochaeta sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 3.7
-
 ATP pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
2.7.2.1 3.8
-
 ATP pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
2.7.2.1 11.7
-
 acetate pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
2.7.2.1 38.5
-
 acetate pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
2.7.2.1 40
-
 propionate pH 7.5, 30°C, isoenzyme I Spirochaeta sp.
2.7.2.1 133
-
 propionate pH 7.5, 30°C, isoenzyme II Spirochaeta sp.
2.7.2.14 1.8
-
 ATP pH 7.5, 30°C Spirochaeta sp.
2.7.2.14 4.3
-
 Isobutyrate pH 7.5, 30°C Spirochaeta sp.
2.7.2.14 9.5
-
 Isovalerate pH 7.5, 30°C Spirochaeta sp.
2.7.2.14 10.8
-
 2-methylbutyrate pH 7.5, 30°C Spirochaeta sp.
2.7.2.14 12.5
-
 valerate pH 7.5, 30°C Spirochaeta sp.
2.7.2.14 14.3
-
 propionate pH 7.5, 30°C Spirochaeta sp.
2.7.2.14 16.9
-
 Butyrate pH 7.5, 30°C Spirochaeta sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Ca2+ isoenzyme I Spirochaeta sp.
2.7.2.1 Co2+
-
 Spirochaeta sp.
2.7.2.1 Cu2+
-
 Spirochaeta sp.
2.7.2.1 Mg2+
-
 Spirochaeta sp.
2.7.2.1 Mn2+
-
 Spirochaeta sp.
2.7.2.1 Zn2+
-
 Spirochaeta sp.
2.7.2.14 Co2+ 65% of the activity with Mn2+ Spirochaeta sp.
2.7.2.14 Cu2+ 58% of the activity with Mn2+ Spirochaeta sp.
2.7.2.14 Mg2+ 96% of the activity with Mn2+ Spirochaeta sp.
2.7.2.14 Mn2+ divalent cation required, highest activity with Mn2+ Spirochaeta sp.
2.7.2.14 Zn2+ 42% of the activity with Mn2+ Spirochaeta sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 110000
-
 sucrose density gradient centrifugation, isoenzyme I and II Spirochaeta sp.
2.7.2.1 125000
-
 gel filtration, isoenzyme I and II Spirochaeta sp.
2.7.2.14 76000
-
 gel filtration Spirochaeta sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Spirochaeta sp.
-
 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Spirochaeta sp. MA-2
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Spirochaeta sp.
-
-
-
2.7.2.1 Spirochaeta sp. MA-2
-
-
-
2.7.2.14 Spirochaeta sp.
-
 MA-2
-
2.7.2.14 Spirochaeta sp. MA-2
-
 MA-2
-

Storage Stability

EC Number Storage Stability Organism
2.7.2.14 -50°C, crude cell extract stable for 2 months Spirochaeta sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Spirochaeta sp. ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Spirochaeta sp. MA-2 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + propionate
-
 Spirochaeta sp. ADP + propionyl phosphate
-
 r
2.7.2.1 CTP + acetate
-
 Spirochaeta sp. CDP + acetyl phosphate
-
 r
2.7.2.1 CTP + acetate
-
 Spirochaeta sp. MA-2 CDP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Spirochaeta sp. GDP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Spirochaeta sp. MA-2 GDP + acetyl phosphate
-
 r
2.7.2.1 ITP + acetate
-
 Spirochaeta sp. IDP + acetyl phosphate
-
 r
2.7.2.1 ITP + acetate
-
 Spirochaeta sp. MA-2 IDP + acetyl phosphate
-
 r
2.7.2.1 additional information isobutyrate, isovalerate, 2-methylbutyrate, butyrate and valerate are no substrates Spirochaeta sp. ?
-
 ?
2.7.2.1 additional information isobutyrate, isovalerate, 2-methylbutyrate, butyrate and valerate are no substrates Spirochaeta sp. MA-2 ?
-
 ?
2.7.2.1 UTP + acetate
-
 Spirochaeta sp. UDP + acetyl phosphate
-
 r
2.7.2.14 ATP + 2-methylbutanoate
-
 Spirochaeta sp. ADP + 2-methylbutanoyl phosphate
-
 ?
2.7.2.14 ATP + butanoate
-
 Spirochaeta sp. ADP + butanoyl phosphate
-
 ?
2.7.2.14 ATP + isobutanoate
-
 Spirochaeta sp. ADP + isobutanoyl phosphate
-
 ?
2.7.2.14 ATP + isopentanoate
-
 Spirochaeta sp. ADP + isopentanoyl phosphate
-
 ?
2.7.2.14 ATP + pentanoate
-
 Spirochaeta sp. ADP + pentanoyl phosphate
-
 ?
2.7.2.14 ATP + propionate
-
 Spirochaeta sp. ADP + propanoyl phosphate
-
 ?
2.7.2.14 CTP + isobutanoate 103% of the activity with ATP Spirochaeta sp. CDP + isobutanoyl phosphate
-
 ?
2.7.2.14 GTP + isobutanoate 70% of the activity with ATP Spirochaeta sp. GDP + isobutanoyl phosphate
-
 ?
2.7.2.14 ITP + isobutanoate 68% of the activity with ATP Spirochaeta sp. IDP + isobutanoyl phosphate
-
 ?
2.7.2.14 additional information no actrivity with acetate Spirochaeta sp. ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.14 45
-
 30 min, 25% loss of activity Spirochaeta sp.
2.7.2.14 60
-
 30 min, complete loss of activity Spirochaeta sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.14 7.2
-
-
 Spirochaeta sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.14 6 8.5 more than 80% of maximal activity at pH 6.0 and 8.5 Spirochaeta sp.