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Literature summary extracted from

  • Nakajima, H.; Suzuki, K.; Imahori, K.
    Purification and properties of acetate kinase from Bacillus stearothermophilus (1978), J. Biochem., 84, 193-203.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.1
-
 Geobacillus stearothermophilus

General Stability

EC Number General Stability Organism
2.7.2.1 ATP or ADP protects the enzyme from inactivation Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 2,4,6-Trinitrobenzene sulfonic acid
-
 Geobacillus stearothermophilus
2.7.2.1 additional information not inactivated by 5,5'-dithiobis(2-nitrobenzoic acid), tetranitromethane or 2-hydroxy-3-nitro-benzyl bromide Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.2.1 Ca2+
-
 Geobacillus stearothermophilus
2.7.2.1 Co2+
-
 Geobacillus stearothermophilus
2.7.2.1 Mg2+
-
 Geobacillus stearothermophilus
2.7.2.1 Mn2+
-
 Geobacillus stearothermophilus
2.7.2.1 Ni2+
-
 Geobacillus stearothermophilus
2.7.2.1 Zn2+
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.1 43000
-
 4 * 43000, SDS-PAGE Geobacillus stearothermophilus
2.7.2.1 160000
-
 gel filtration Geobacillus stearothermophilus
2.7.2.1 170000
-
 sedimentation analysis Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.2.1 ATP + acetate Geobacillus stearothermophilus
-
 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate Geobacillus stearothermophilus NCA 1503
-
 ADP + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Geobacillus stearothermophilus
-
 NCA 1503
-
2.7.2.1 Geobacillus stearothermophilus NCA 1503
-
 NCA 1503
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.7.2.1 sensitive to light oxidation Geobacillus stearothermophilus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.1
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.1 1.71
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 ATP + acetate
-
 Geobacillus stearothermophilus ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Geobacillus stearothermophilus NCA 1503 ADP + acetyl phosphate
-
 r
2.7.2.1 ATP + propionate
-
 Geobacillus stearothermophilus ADP + propionyl phosphate
-
 r
2.7.2.1 CTP + acetate
-
 Geobacillus stearothermophilus CDP + acetyl phosphate
-
 r
2.7.2.1 CTP + acetate
-
 Geobacillus stearothermophilus NCA 1503 CDP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Geobacillus stearothermophilus GDP + acetyl phosphate
-
 r
2.7.2.1 GTP + acetate
-
 Geobacillus stearothermophilus NCA 1503 GDP + acetyl phosphate
-
 r
2.7.2.1 additional information formate is no substrate Geobacillus stearothermophilus ?
-
 ?
2.7.2.1 additional information formate is no substrate Geobacillus stearothermophilus NCA 1503 ?
-
 ?
2.7.2.1 UTP + acetate
-
 Geobacillus stearothermophilus UDP + acetyl phosphate
-
 r
2.7.2.1 UTP + acetate
-
 Geobacillus stearothermophilus NCA 1503 UDP + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.7.2.1 tetramer 4 * 43000, SDS-PAGE Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.1 65
-
 quite stable up to Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.2.1 7.3
-
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.2.1 6 9.5
-
 Geobacillus stearothermophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.2.1 6 11 rather stable at alkaline pH but rapidly inactivated at acidic pH lower than 6.0, about 40% loss of activity at pH 11.0 Geobacillus stearothermophilus