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Literature summary extracted from

  • Stepp, L.R.; Pettit, F.H.; Yeaman, S.J.; Reed, L.J.
    Purification and properties of pyruvate dehydrogenase kinase from bovine kidney (1983), J. Biol. Chem., 258, 9454-9458.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.2 dihydrolipoyl transacetylase
-
 Bos taurus

General Stability

EC Number General Stability Organism
2.7.11.2 labile to freeze-thawing Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.2 chymotrypsin proteolysis of kinase alpha-, not beta-subunit, no inactivation by trypsin-mediated proteolysis of beta-subunit Bos taurus
2.7.11.2 additional information no inhibition by cAMP; no inhibition by cGMP; not affected by calmodulin with or without Ca2+ Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.2 mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.2 Mg2+ requirement Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.2 45000
-
 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
2.7.11.2 48000
-
 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus beta-subunit harbors a regulatory role ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.2 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.2 2700fold to homogeneity Bos taurus
2.7.11.2 kidney, from highly purifed pyruvate dehydrogenase-complex Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.2 kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.2 0.33
-
-
 Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.11.2 4°C, 0.02% NaN3, t1/2: 1 month Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] highly specific for the substrate Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] beta-subunit harbors a regulatory role Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 additional information little, if any activity with casein of bovine kidney Bos taurus ?
-
 ?
2.7.11.2 additional information no activity with histones of calf thymus type II-A, VI-S and VIII-S Bos taurus ?
-
 ?
2.7.11.2 additional information no activity with glycogen synthase a and rabbit skeletal muscle phosphorylase b Bos taurus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.2 dimer 1 * 48000, alpha + 1 * 45000, beta, SDS-PAGE Bos taurus
2.7.11.2 More kinase activity resides in alpha-subunit Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.2 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.2 7
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.2 ATP dependent on Bos taurus
2.7.11.2 additional information no activation by cAMP Bos taurus
2.7.11.2 additional information no activation by Ca2+/calmodulin or calmodulin alone Bos taurus
2.7.11.2 additional information no activation by cGMP Bos taurus