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Literature summary extracted from

  • Pratt, T.E.; Roche, T.E.
    Mechanism of pyruvate inhibition of kidney pyruvate dehydrogenase kinase and synergistic inhibition by pyruvate and ADP (1979), J. Biol. Chem., 254, 7191-7196.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.2 Dichloroacetate noncompetitive; pyruvate analog, synergism with ADP, K+ or phosphate, kinetics Bos taurus
2.7.11.2 HPO42- enhances inhibition by pyruvate or dichloroacetate Bos taurus
2.7.11.2 K+ pyruvate or dichloroacetate Bos taurus
2.7.11.2 pyruvate kinetics; noncompetitive to ATP; synergism with ADP; synergism with K+ or phosphate Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.2 0.025
-
 ATP
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.2 Mg2+ requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] Bos taurus catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.2 Bos taurus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.11.2 ATP + [pyruvate dehydrogenase (acetyl-transferring)] = ADP + [pyruvate dehydrogenase (acetyl-transferring)] phosphate mechanism Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.2 kidney
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)]
-
 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir
2.7.11.2 ATP + [pyruvate dehydrogenase (lipoamide)] catalyzes inactivation through phosphorylation of pyruvate dehydrogenase complex EC 1.2.4.1 Bos taurus ADP + [pyruvate dehydrogenase (lipoamide)] phosphate
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.2 30
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.2 7.2
-
 assay at Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.2 ATP dependent on Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.11.2 0.27
-
 pyruvate pH 7.2, 30°C Bos taurus
2.7.11.2 0.27
-
 Dichloroacetate pH 7.2, 30°C Bos taurus
2.7.11.2 0.27
-
 pyruvate kinetic constant from binding study Bos taurus
2.7.11.2 0.27
-
 Dichloroacetate kinetic constant from binding study Bos taurus