Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reeves, R.E.; Lobelle-Rich, P.; Eubank, W.B.
    6-Phosphofructokinase (pyrophosphate) from Entamoeba histolytica (1982), Methods Enzymol., 90, 97-102.
No PubMed abstract available

General Stability

EC Number General Stability Organism
2.7.1.90 unstable to freezing in 20 mM imidazole buffer Entamoeba histolytica

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 Ca2+
-
 Entamoeba histolytica
2.7.1.90 Ni2+
-
 Entamoeba histolytica
2.7.1.90 Zn2+
-
 Entamoeba histolytica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.014 0.017 diphosphate
-
 Entamoeba histolytica
2.7.1.90 0.018 0.023 D-fructose 1,6-bisphosphate
-
 Entamoeba histolytica
2.7.1.90 0.038
-
 D-fructose 6-phosphate
-
 Entamoeba histolytica
2.7.1.90 0.06
-
 sedoheptulose 7-phosphate
-
 Entamoeba histolytica
2.7.1.90 0.59 1 phosphate
-
 Entamoeba histolytica
2.7.1.90 3.6
-
 arsenate with D-fructose 1,6-bisphosphate Entamoeba histolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 divalent cations required Entamoeba histolytica
2.7.1.90 divalent cations Mn2+ or Mg2+ Entamoeba histolytica
2.7.1.90 Mg2+ requirement Entamoeba histolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 83000
-
 gel filtration Entamoeba histolytica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Entamoeba histolytica
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Entamoeba histolytica K-9 / ATCC 30015
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Entamoeba histolytica
-
-
-
2.7.1.90 Entamoeba histolytica K-9 / ATCC 30015
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90
-
 Entamoeba histolytica

Storage Stability

EC Number Storage Stability Organism
2.7.1.90 -20°C, in salt-containing solution, 25% glycerol, t1/2: 1 year Entamoeba histolytica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 arsenate + D-fructose 1,6-bisphosphate
-
 Entamoeba histolytica ?
-
 r
2.7.1.90 arsenate + D-fructose 1,6-bisphosphate
-
 Entamoeba histolytica K-9 / ATCC 30015 ?
-
 r
2.7.1.90 diphosphate + 2,5-anhydro-D-mannitol 6-phosphate
-
 Entamoeba histolytica phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + 2,5-anhydro-D-mannitol 6-phosphate
-
 Entamoeba histolytica K-9 / ATCC 30015 phosphate + 2,5-anhydro-D-mannitol 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Entamoeba histolytica phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Entamoeba histolytica phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Entamoeba histolytica K-9 / ATCC 30015 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Entamoeba histolytica K-9 / ATCC 30015 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-sedoheptulose 7-phosphate
-
 Entamoeba histolytica phosphate + D-sedoheptulose 1,7-bisphosphate
-
 r
2.7.1.90 diphosphate + D-sedoheptulose 7-phosphate
-
 Entamoeba histolytica K-9 / ATCC 30015 phosphate + D-sedoheptulose 1,7-bisphosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.90 additional information
-
 additional information
-
 Entamoeba histolytica