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Literature summary extracted from

  • Kombrink, E.; Kruger, N.J.; Beevers, H.
    Kinetic properties of pyrophosphate:D-fructose-6-phosphate phosphotransferase from germinating castor bean endosperm (1984), Plant Physiol., 74, 395-401.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 D-fructose 2,6-bisphosphate 20fold activation of forward reaction, 10fold increase in affinity for the substrates of both reaction directions Ricinus communis

General Stability

EC Number General Stability Organism
2.7.1.90 glycerol essential for stability during purification Ricinus communis
2.7.1.90 Mg2+ stabilizes Ricinus communis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 Ca2+
-
Ricinus communis
2.7.1.90 Cl- at high concentrations Ricinus communis
2.7.1.90 D-fructose 1,6-bisphosphate
-
Ricinus communis
2.7.1.90 D-fructose 6-phosphate
-
Ricinus communis
2.7.1.90 diphosphate
-
Ricinus communis
2.7.1.90 Mg2+ at high concentrations Ricinus communis
2.7.1.90 NH4+ weak Ricinus communis
2.7.1.90 Ni2+
-
Ricinus communis
2.7.1.90 NO3- at high concentrations Ricinus communis
2.7.1.90 phosphate inhibitory in the reverse reaction; in the forward reaction only in presence of fructose 2,6-bisphosphate Ricinus communis
2.7.1.90 SO42- at high concentrations Ricinus communis
2.7.1.90 sulfate weak Ricinus communis
2.7.1.90 Zn2+
-
Ricinus communis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.014 0.017 diphosphate pH 7.5, 25°C Ricinus communis
2.7.1.90 0.023
-
fructose 1,6-bisphosphate reverse reaction, pH 7.8, 25°C Ricinus communis
2.7.1.90 0.3
-
D-fructose 6-phosphate forward reaction, pH 7.5, 25°C Ricinus communis
2.7.1.90 0.63
-
phosphate pH 7.8, 25°C Ricinus communis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Co2+ less effective than Mg2+ Ricinus communis
2.7.1.90 divalent cations required Ricinus communis
2.7.1.90 divalent cations Mn2+ or Mg2+ Ricinus communis
2.7.1.90 Mg2+ requirement Ricinus communis
2.7.1.90 Mg2+ inhibitory at high concentration Ricinus communis
2.7.1.90 Mn2+ requirement Ricinus communis
2.7.1.90 Mn2+ less effective than Mg2+ Ricinus communis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Ricinus communis
-
phosphate + D-fructose 1,6-bisphosphate
-
r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Ricinus communis
-
var. Hale
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 573fold Ricinus communis
2.7.1.90 partial Ricinus communis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.90 endosperm
-
Ricinus communis
-
2.7.1.90 seed germinating Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 8.25
-
purified enzyme Ricinus communis

Storage Stability

EC Number Storage Stability Organism
2.7.1.90 4°C, in glycerol, 20% w/v, 20% loss of activity within 1 week Ricinus communis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
Ricinus communis phosphate + D-fructose 1,6-bisphosphate
-
r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Ricinus communis phosphate + D-fructose 1,6-bisphosphate
-
r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 25
-
assay at Ricinus communis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7.3 7.7 forward reaction Ricinus communis
2.7.1.90 7.8
-
reverse reaction Ricinus communis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.90 0.0025
-
diphosphate forward reaction, pH 7.5, 25°C Ricinus communis
2.7.1.90 0.013
-
D-fructose 1,6-bisphosphate reverse reaction, pH 7.8, 25°C Ricinus communis
2.7.1.90 0.113
-
D-fructose 6-phosphate forward reaction, pH 7.5, 25°C Ricinus communis
2.7.1.90 0.39
-
phosphate reverse reaction, pH 7.8, 25°C Ricinus communis