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Literature summary extracted from

  • Pfleiderer, C.; Klemme, J.H.
    Pyrophosphate-dependent D-D-fructose-6-phosphate-phosphotransferase in Rhodospirillaceae (1980), Z. Naturforsch. C, 35c, 229-238.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 ATP slight activation of forward reaction Rhodospirillum rubrum
2.7.1.90 cAMP forward reaction Rhodospirillum rubrum
2.7.1.90 citrate slight activation of forward reaction Rhodospirillum rubrum

General Stability

EC Number General Stability Organism
2.7.1.90 PAGE or dialysis against Mg-free Tris-HCl buffer, pH 7.5, inactivates Rhodospirillum rubrum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 ADP kinetics Rhodospirillum rubrum
2.7.1.90 AMP kinetics Rhodospirillum rubrum
2.7.1.90 AMP kinetics Rubrivivax gelatinosus
2.7.1.90 ATP only at low concentrations of MgCl2, reverse reaction Rhodospirillum rubrum
2.7.1.90 citrate
-
 Rhodospirillum rubrum
2.7.1.90 D-glucose 6-phosphate weak Rhodospirillum rubrum
2.7.1.90 D-glucose 6-phosphate weak Rubrivivax gelatinosus
2.7.1.90 diphosphate reverse reaction Rhodospirillum rubrum
2.7.1.90 additional information no inhibition by cAMP Rhodospirillum rubrum
2.7.1.90 phosphoenolpyruvate weak, reverse reaction Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 0.018 0.023 D-fructose 1,6-bisphosphate pH 8.6, 30°C Rhodospirillum rubrum
2.7.1.90 0.025 0.033 diphosphate pH 7.2, 30°C Rhodospirillum rubrum
2.7.1.90 0.3 0.38 D-fructose 6-phosphate pH 7.2, 30°C Rhodospirillum rubrum
2.7.1.90 0.59 1 phosphate pH 8.6, 30°C Rhodospirillum rubrum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Co2+ no activitaion Rhodospirillum rubrum
2.7.1.90 Mg2+ requirement Rubrivivax gelatinosus
2.7.1.90 Mg2+ requirement Rhodospirillum rubrum
2.7.1.90 Mn2+ not required Rhodospirillum rubrum
2.7.1.90 additional information no activation by Zn2+ Rhodospirillum rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 95000
-
 gel filtration Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Rubrivivax gelatinosus
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate Rhodospirillum rubrum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate Rhodospirillum rubrum S1
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 no activity in Rhodopseudomonas capsulata
-
-
-
2.7.1.90 no activity in Rhodopseudomonas capsulata Kb1
-
-
-
2.7.1.90 no activity in Rhodopseudomonas palustris
-
-
-
2.7.1.90 no activity in Rhodopseudomonas palustris 1a1
-
-
-
2.7.1.90 no activity in Rhodopseudomonas sphaeroides
-
-
-
2.7.1.90 no activity in Rhodopseudomonas viridis
-
-
-
2.7.1.90 Rhodospirillum rubrum
-
-
-
2.7.1.90 Rhodospirillum rubrum S1
-
-
-
2.7.1.90 Rubrivivax gelatinosus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 partial 83.4fold Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 24.2
-
 partially purified enzyme Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Rubrivivax gelatinosus phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Rhodospirillum rubrum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Rubrivivax gelatinosus phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Rhodospirillum rubrum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Rubrivivax gelatinosus phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Rhodospirillum rubrum phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Rhodospirillum rubrum S1 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Rhodospirillum rubrum S1 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Rhodospirillum rubrum S1 phosphate + D-fructose 1,6-bisphosphate
-
 r
2.7.1.90 phosphate + D-glucose 1,6-bisphosphate reaction at about 70% of the rate with D-fructose 1,6-bisphosphate Rhodospirillum rubrum ?
-
 ?
2.7.1.90 phosphate + D-glucose 1,6-bisphosphate reaction at about 70% of the rate with D-fructose 1,6-bisphosphate Rhodospirillum rubrum S1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 30
-
 assay at Rhodospirillum rubrum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.90 62
-
 10 min stable in the presence of 5 mM MgCl2 Rhodospirillum rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7.2
-
 forward reaction Rhodospirillum rubrum
2.7.1.90 8.6
-
 reverse reaction Rhodospirillum rubrum