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Literature summary extracted from

  • Bertagnolli, B.L.; Cook, P.F.
    Kinetic mechanism of pyrophosphate-dependent phosphofructokinase from Propionibacterium freudenreichii (1984), Biochemistry, 23, 4101-4108.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 2,5-Anhydro-D-mannitol 1,6-bisphosphate D-fructose 1,6-bisphosphate-analog, kinetics Propionibacterium freudenreichii
2.7.1.90 D-fructose 1,6-bisphosphate forward reaction, product inhibition, kinetics Propionibacterium freudenreichii
2.7.1.90 D-fructose 6-phosphate reverse reaction, product inhibition, kinetics Propionibacterium freudenreichii
2.7.1.90 D-tagatose 6-phosphate kinetics, dead-end substrate Propionibacterium freudenreichii
2.7.1.90 diphosphate
-
 Propionibacterium freudenreichii
2.7.1.90 magnesium diphosphate reverse reaction, product inhibition, kinetics Propionibacterium freudenreichii
2.7.1.90 Methylenediphosphonate kinetics, diphosphate analog Propionibacterium freudenreichii
2.7.1.90 phosphate forward reaction, product inhibition, kinetics Propionibacterium freudenreichii
2.7.1.90 SO42- phosphate analog, kinetics Propionibacterium freudenreichii
2.7.1.90 Tetrapolyphosphate
-
 Propionibacterium freudenreichii
2.7.1.90 tripolyphosphate
-
 Propionibacterium freudenreichii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 additional information
-
 additional information kinetics Propionibacterium freudenreichii
2.7.1.90 0.005
-
 diphosphate as Mg2+ complex, pH 8.0, 25°C Propionibacterium freudenreichii
2.7.1.90 0.03
-
 D-fructose 1,6-bisphosphate reverse reaction, pH 8.0, 25°C Propionibacterium freudenreichii
2.7.1.90 0.1
-
 Mg2+ reverse reaction, pH 8.0, 25°C Propionibacterium freudenreichii
2.7.1.90 0.13
-
 D-fructose 6-phosphate pH 8.0, 25°C Propionibacterium freudenreichii
2.7.1.90 0.56
-
 phosphate pH 8.0, 25°C Propionibacterium freudenreichii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Mg2+ requirement Propionibacterium freudenreichii
2.7.1.90 Mg2+ magnesium diphosphate is the actual substrate of forward reaction Propionibacterium freudenreichii
2.7.1.90 Mg2+ product inhibition of reverse reaction, kinetics Propionibacterium freudenreichii
2.7.1.90 Mg2+ mainly required for the forward reaction Propionibacterium freudenreichii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Propionibacterium freudenreichii
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Propionibacterium freudenreichii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 to apparent homogeneity Propionibacterium freudenreichii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.90 diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate random terreactant or rapid equilibrium random bi-ter mechanism Propionibacterium freudenreichii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Propionibacterium freudenreichii phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Propionibacterium freudenreichii phosphate + D-fructose 1,6-bisphosphate
-
 r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 25
-
 assay at Propionibacterium freudenreichii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 8
-
 assay at Propionibacterium freudenreichii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.90 additional information
-
 additional information inhibition kinetics: inhibition by product and inhibitors Propionibacterium freudenreichii
2.7.1.90 0.023
-
 diphosphate versus phosphate, pH 8.0, 25°C Propionibacterium freudenreichii
2.7.1.90 0.086
-
 D-fructose 1,6-bisphosphate versus D-fructose 6-phosphate, pH 8.0, 25°C Propionibacterium freudenreichii
2.7.1.90 0.62
-
 D-fructose 6-phosphate versus D-fructose 1,6-bisphosphate, pH 8.0, 25°C Propionibacterium freudenreichii
2.7.1.90 10.3
-
 phosphate versus diphosphate, pH 8.0, 25°C Propionibacterium freudenreichii