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Literature summary extracted from

  • Pollack, J.D.; Williams, M.V.
    PPi-dependent phosphofructotransferase (phosphofructokinase) activity in the mollicutes (mycoplasma) Acholeplasma laidlawii (1986), J. Bacteriol., 165, 53-60.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.90 citrate no activation Acholeplasma laidlawii
2.7.1.90 D-fructose 2,6-bisphosphate no activation Acholeplasma laidlawii
2.7.1.90 additional information no activation by or phosphoenolpyruvate, AMP, GDP, citrate, and ammonium sulfate Acholeplasma laidlawii
2.7.1.90 additional information no activation by ATP Acholeplasma laidlawii
2.7.1.90 additional information no activation by polyphosphate, nucleoside triphosphates Acholeplasma laidlawii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.90 ADP no inhibition Acholeplasma laidlawii
2.7.1.90 AMP no inhibition Acholeplasma laidlawii
2.7.1.90 AMP no inhibition Mesoplasma florum
2.7.1.90 citrate no inhibition Acholeplasma laidlawii
2.7.1.90 citrate no inhibition Mesoplasma florum
2.7.1.90 Co2+ inhibitory at high concentration Acholeplasma laidlawii
2.7.1.90 Mn2+ inhibitory at high concentration Acholeplasma laidlawii
2.7.1.90 additional information no inhibition by ammonium sulfate, D-fructose 2,6-bisphosphate, GDP; no inhibition by nucleoside triphosphates Acholeplasma laidlawii
2.7.1.90 phosphoenolpyruvate no inhibition Acholeplasma laidlawii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.90 additional information
-
 additional information kinetics Acholeplasma laidlawii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.90 cytosol
-
 Acholeplasma laidlawii 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.90 Co2+ less effective than Mg2+ Acholeplasma laidlawii
2.7.1.90 Co2+ less effective than Mn2+ Acholeplasma laidlawii
2.7.1.90 Co2+ inhibitory at high concentration Acholeplasma laidlawii
2.7.1.90 divalent cations required Acholeplasma laidlawii
2.7.1.90 divalent cations descending order Mg2+, Mn2+ and Co2+ Acholeplasma laidlawii
2.7.1.90 divalent cations Mn2+ or Mg2+ Acholeplasma laidlawii
2.7.1.90 Mg2+ requirement Acholeplasma laidlawii
2.7.1.90 Mg2+ requirement Mesoplasma florum
2.7.1.90 Mn2+ requirement Acholeplasma laidlawii
2.7.1.90 Mn2+ requirement Mesoplasma florum
2.7.1.90 Mn2+ less effective than Mg2+ Acholeplasma laidlawii
2.7.1.90 Mn2+ inhibitory at high concentration Acholeplasma laidlawii
2.7.1.90 additional information no activation by Zn2+ Acholeplasma laidlawii
2.7.1.90 additional information no activation by (NH4)2SO4 Acholeplasma laidlawii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.90 74000
-
 sucrose density gradient centrifugation Acholeplasma laidlawii
2.7.1.90 81000
-
 PAGE Acholeplasma laidlawii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.90 diphosphate + D-fructose 6-phosphate Acholeplasma laidlawii
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Mesoplasma florum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Mesoplasma florum L1
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate Acholeplasma laidlawii B-PG9
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.90 Acholeplasma laidlawii
-
-
-
2.7.1.90 Acholeplasma laidlawii B-PG9
-
-
-
2.7.1.90 Mesoplasma florum
-
-
-
2.7.1.90 Mesoplasma florum L1
-
-
-
2.7.1.90 no activity in Mycoplasma gallisepticum S6
-
-
-
2.7.1.90 no activity in Spiroplasma floricola
-
-
-
2.7.1.90 no activity in Spiroplasma floricola 23-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.90 partial, 430fold Acholeplasma laidlawii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.90 0.00016
-
 crude enzyme extract, phosphor donor in the forward reaction is diphosphate Mesoplasma florum
2.7.1.90 0.00021
-
 crude enzyme extract, phosphor donor in the forward reaction is ATP Mesoplasma florum
2.7.1.90 12
-
 partially purified enzyme Acholeplasma laidlawii

Storage Stability

EC Number Storage Stability Organism
2.7.1.90 -20°C, in kappa-buffer with glycerol, 10% v/v, about 25% loss of activity within 6 months Acholeplasma laidlawii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.90 arsenate + D-fructose 1,6-bisphosphate specific for phosphate or arsenate in the reverse reaction Acholeplasma laidlawii ?
-
 r
2.7.1.90 arsenate + D-fructose 1,6-bisphosphate specific for phosphate or arsenate in the reverse reaction Acholeplasma laidlawii B-PG9 ?
-
 r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Acholeplasma laidlawii phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Mesoplasma florum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate phosphate cannot replace diphosphate and vice versa Mesoplasma florum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate phosphate cannot replace diphosphate and vice versa Acholeplasma laidlawii phosphate + D-fructose 1,6-bisphosphate arsenate can replace phosphate in the reverse reaction r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Mesoplasma florum phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Acholeplasma laidlawii phosphate + D-fructose 1,6-bisphosphate arsenate can replace phosphate in the reverse reaction r
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Mesoplasma florum L1 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate phosphate cannot replace diphosphate and vice versa Mesoplasma florum L1 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Mesoplasma florum L1 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate
-
 Acholeplasma laidlawii B-PG9 phosphate + D-fructose 1,6-bisphosphate
-
 ?
2.7.1.90 diphosphate + D-fructose 6-phosphate phosphate cannot replace diphosphate and vice versa Acholeplasma laidlawii B-PG9 phosphate + D-fructose 1,6-bisphosphate arsenate can replace phosphate in the reverse reaction r
2.7.1.90 diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Acholeplasma laidlawii B-PG9 phosphate + D-fructose 1,6-bisphosphate arsenate can replace phosphate in the reverse reaction r

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.90 21 22 assay at Acholeplasma laidlawii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.90 70
-
 2 min, about 60% loss of activity, inactivation within 10 min Acholeplasma laidlawii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.90 7.4
-
-
 Acholeplasma laidlawii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.90 3.5 10.3 smooth hyperbolic pH-value/activity curve Acholeplasma laidlawii