EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.90 | ADP | weak | Entamoeba histolytica | |
2.7.1.90 | Ca2+ | - |
Entamoeba histolytica | |
2.7.1.90 | D-fructose 6-phosphate | competitive versus D-fructose 1,6-bisphosphate and phosphate | Entamoeba histolytica | |
2.7.1.90 | diphosphate | - |
Entamoeba histolytica | |
2.7.1.90 | EDTA | - |
Entamoeba histolytica | |
2.7.1.90 | Ni2+ | - |
Entamoeba histolytica | |
2.7.1.90 | phosphate | competitive versus diphosphate | Entamoeba histolytica | |
2.7.1.90 | Zn2+ | - |
Entamoeba histolytica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.90 | 0.006 | - |
diphosphate | pH 7.0 | Entamoeba histolytica | |
2.7.1.90 | 0.008 | - |
Mg2+ | forward reaction, pH 7.0, 30°C | Entamoeba histolytica | |
2.7.1.90 | 0.009 | - |
D-fructose 1,6-bisphosphate | pH 7.0, 30°C | Entamoeba histolytica | |
2.7.1.90 | 0.5 | - |
Mg2+ | reverse reaction, pH 7.0, 30°C | Entamoeba histolytica | |
2.7.1.90 | 0.59 | 1 | phosphate | - |
Entamoeba histolytica | |
2.7.1.90 | 3.6 | - |
arsenate | pH 7.0, 30°C | Entamoeba histolytica | |
2.7.1.90 | 3.6 | - |
arsenate | with D-fructose 1,6-bisphosphate | Entamoeba histolytica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.90 | Co2+ | less effective than Mg2+ | Entamoeba histolytica | |
2.7.1.90 | Co2+ | less effective than Mn2+ | Entamoeba histolytica | |
2.7.1.90 | divalent cations | required | Entamoeba histolytica | |
2.7.1.90 | divalent cations | desecending order Mn2+, Mg2+, Co2+ | Entamoeba histolytica | |
2.7.1.90 | Mg2+ | requirement | Entamoeba histolytica | |
2.7.1.90 | Mg2+ | less effective than Mn2+ | Entamoeba histolytica | |
2.7.1.90 | Mg2+ | enzyme requires 0.5 mM in the forward reaction, 0.0008 mM in the reverse reaction | Entamoeba histolytica | |
2.7.1.90 | Mn2+ | requirement | Entamoeba histolytica | |
2.7.1.90 | additional information | no activation by Ni2+ | Entamoeba histolytica | |
2.7.1.90 | additional information | no activation by Zn2+ | Entamoeba histolytica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.90 | 83000 | - |
gel filtration | Entamoeba histolytica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.90 | diphosphate + D-fructose 6-phosphate | Entamoeba histolytica | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.90 | diphosphate + D-fructose 6-phosphate | Entamoeba histolytica DKB | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.90 | Entamoeba histolytica | - |
DKB-strain | - |
2.7.1.90 | Entamoeba histolytica DKB | - |
DKB-strain | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.90 | partial | Entamoeba histolytica |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.90 | diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate | random bi bi mechanism | Entamoeba histolytica |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.90 | 40 | - |
partially purified enzyme | Entamoeba histolytica |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.1.90 | -20°C, partially purified enzyme, loss of 25% activity after 1 month | Entamoeba histolytica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.90 | arsenate + D-fructose 1,6-bisphosphate | - |
Entamoeba histolytica | ? | - |
r | |
2.7.1.90 | arsenate + D-fructose 1,6-bisphosphate | - |
Entamoeba histolytica DKB | ? | - |
r | |
2.7.1.90 | diphosphate + D-fructose 6-phosphate | - |
Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.90 | diphosphate + D-fructose 6-phosphate | no phosphate group exchange reaction between phosphate and diphosphate, or D-fructose 6-phosphate and D-fructose 1,6-bisphosphate in absence of a third substrate | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
2.7.1.90 | diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
2.7.1.90 | diphosphate + D-fructose 6-phosphate | - |
Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
2.7.1.90 | diphosphate + D-fructose 6-phosphate | no phosphate group exchange reaction between phosphate and diphosphate, or D-fructose 6-phosphate and D-fructose 1,6-bisphosphate in absence of a third substrate | Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r | |
2.7.1.90 | diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica DKB | phosphate + D-fructose 1,6-bisphosphate | arsenate can replace phosphate in the reverse reaction | r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.90 | 30 | - |
assay at | Entamoeba histolytica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.90 | 7 | - |
assay at | Entamoeba histolytica |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.90 | 0.115 | - |
D-fructose 6-phosphate | versus diphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
2.7.1.90 | 0.16 | - |
D-fructose 6-phosphate | versus D-fructose 1,6-bisphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
2.7.1.90 | 0.85 | - |
phosphate | versus diphosphate, pH 7.0, 30°C | Entamoeba histolytica | |
2.7.1.90 | 3 | - |
phosphate | versus D-fructose 1,6-bisphosphate, pH 7.0, 30°C | Entamoeba histolytica |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.7.1.90 | Entamoeba histolytica | - |
- |
5.8 |