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Literature summary extracted from

  • Iwahashi, Y.; Hitoshio, A.; Tajima, N.; Nakamura, T.
    Characterization of NADH kinase from Saccharomyces cerevisiae (1989), J. Biochem., 105, 588-593.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.86 acetate activates Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.86 3-acetyl-NAD+ 1 mM, 15% inhibition Saccharomyces cerevisiae
2.7.1.86 ATP above 6 mM Saccharomyces cerevisiae
2.7.1.86 iodoacetic acid 5 mM, 87% inhibition, NADH protects Saccharomyces cerevisiae
2.7.1.86 NEM 5 mM, 77% inhibition, NADH protects, ATP not Saccharomyces cerevisiae
2.7.1.86 PCMB 5 mM, 49% inhibition Saccharomyces cerevisiae
2.7.1.86 phosphoenolpyruvate 30 mM, 57% inhibition Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.86 0.105
-
 NADH pH 7.4, 30°C Saccharomyces cerevisiae
2.7.1.86 2.1
-
 ATP pH 7.4, 30°C Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.86 mitochondrion localized exclusively in mitochondria Saccharomyces cerevisiae 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.86 160000
-
 gel filtration Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.86 Saccharomyces cerevisiae
-
-
-
2.7.1.86 Saccharomyces cerevisiae X2181-1A
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.86
-
 Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.86 729
-
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.7.1.86 -30°C, 20 mM Tris-HCl buffer, pH 7.8, 0.2 M ammonium sulfate, 20 mM MgCl2, 2 mM EDTA, stable for 1 month or more Saccharomyces cerevisiae
2.7.1.86 0-5°C, 20 mM Tris-HCl buffer, pH 7.8, 0.2 M ammonium sulfate, 20 mM MgCl2, 2 mM EDTA, stable for 7 days Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.86 ATP + NADH specific for NADH Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH NAD+ completely ineffective Saccharomyces cerevisiae ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH specific for NADH Saccharomyces cerevisiae X2181-1A ADP + NADPH
-
 ?
2.7.1.86 ATP + NADH NAD+ completely ineffective Saccharomyces cerevisiae X2181-1A ADP + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.86 More presence of 2 or more subunits of different size Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.86 35
-
 5 min, 35% loss of activity Saccharomyces cerevisiae
2.7.1.86 55
-
 5 min, 95% loss of activity Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.86 8.5
-
-
 Saccharomyces cerevisiae