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Literature summary extracted from

  • Thompson, J.; Lichtenthaler, F.W.; Peters, S.; Pikis, A.
    beta-Glucoside kinase (BglK) from Klebsiella pneumoniae. Purification, properties, and preparative synthesis of 6-phospho-beta-D-glucosides (2002), J. Biol. Chem., 277, 34310-34321.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.85 cloning and high expression of His6BglK in Escherichia coli TOP10 Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.85 D103G catalytically inactive protein Klebsiella pneumoniae
2.7.1.85 D7G catalytically inactive protein Klebsiella pneumoniae
2.7.1.85 G131A catalytically inactive protein Klebsiella pneumoniae
2.7.1.85 G133A catalytically inactive protein Klebsiella pneumoniae
2.7.1.85 G9A catalytically inactive protein Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.85 NEM considerable protection Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.85 0.11
-
n-octyl beta-D-glucopyranoside pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.18
-
Salicin pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.21
-
arbutin pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.24
-
ATP pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.47
-
cellobiose pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.47
-
amygdalin pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.56
-
gentiobiose pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.63
-
laminaribiose pH 7.5 Klebsiella pneumoniae
2.7.1.85 0.65
-
phenyl beta-D-glucopyranoside pH 7.5 Klebsiella pneumoniae
2.7.1.85 1.56
-
cellobiitol pH 7.5 Klebsiella pneumoniae
2.7.1.85 2.03
-
isopropyl beta-D-thioglucopyranoside pH 7.5 Klebsiella pneumoniae
2.7.1.85 2.23
-
methyl beta-D-glucopyranoside pH 7.5 Klebsiella pneumoniae
2.7.1.85 3.15
-
sophorose pH 7.5 Klebsiella pneumoniae
2.7.1.85 10.54
-
thiocellobiose pH 7.5 Klebsiella pneumoniae
2.7.1.85 40.29
-
D-glucose pH 7.5 Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.85 32000
-
x * 32000, SDS-PAGE Listeria monocytogenes
2.7.1.85 32000
-
x * 32000, SDS-PAGE Listeria innocua
2.7.1.85 32697
-
4 * 32697, calculation from nucleotide sequence Klebsiella pneumoniae
2.7.1.85 33000
-
4 * 33000, non-covalently linked subunits, SDS-PAGE Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.85 Klebsiella pneumoniae
-
ATCC 23357
-
2.7.1.85 Listeria innocua
-
CLIP 11262
-
2.7.1.85 Listeria innocua CLIP 11262
-
CLIP 11262
-
2.7.1.85 Listeria monocytogenes
-
EGD-e
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.85 recombinant enzyme Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.85 24
-
-
Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.85 ATP + amygdalin
-
Klebsiella pneumoniae ADP + amygdalin 6-phosphate
-
?
2.7.1.85 ATP + arbutin
-
Klebsiella pneumoniae ADP + arbutin 6-phosphate
-
?
2.7.1.85 ATP + cellobiitol
-
Klebsiella pneumoniae ?
-
?
2.7.1.85 ATP + cellobiose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
2.7.1.85 ATP + cellobiose
-
Listeria monocytogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
2.7.1.85 ATP + cellobiose
-
Listeria innocua ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
2.7.1.85 ATP + cellobiose
-
Listeria innocua CLIP 11262 ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
2.7.1.85 ATP + D-glucose
-
Klebsiella pneumoniae ADP + D-glucose 6-phosphate
-
?
2.7.1.85 ATP + gentiobiose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
-
?
2.7.1.85 ATP + isopropyl beta-D-thioglucopyranoside
-
Klebsiella pneumoniae ADP + isopropyl beta-D-thioglucopyranoside 6-phosphate
-
?
2.7.1.85 ATP + isopropyl beta-D-thioglucopyranoside
-
Klebsiella pneumoniae ?
-
?
2.7.1.85 ATP + laminaribiose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucopyranosyl-(1,3)-D-glucose
-
?
2.7.1.85 ATP + methyl beta-D-glucoside
-
Klebsiella pneumoniae ADP + methyl beta-D-glucoside 6-phosphate
-
?
2.7.1.85 ATP + n-octyl-beta-D-glucopyranoside
-
Klebsiella pneumoniae ADP + n-octyl-beta-D-glucopyranoside 6-phosphate
-
?
2.7.1.85 ATP + phenyl beta-D-glucoside
-
Klebsiella pneumoniae ADP + phenyl beta-D-glucoside 6-phosphate
-
?
2.7.1.85 ATP + salicin
-
Klebsiella pneumoniae ADP + salicin 6-phosphate
-
?
2.7.1.85 ATP + sophorose
-
Klebsiella pneumoniae ADP + 6-phospho-beta-D-glucopyranosyl-(1,2)-D-glucose
-
?
2.7.1.85 ATP + thiocellobiose
-
Klebsiella pneumoniae ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.85 ? x * 32000, SDS-PAGE Listeria monocytogenes
2.7.1.85 ? x * 32000, SDS-PAGE Listeria innocua
2.7.1.85 tetramer 4 * 32697, calculation from nucleotide sequence Klebsiella pneumoniae
2.7.1.85 tetramer 4 * 33000, non-covalently linked subunits, SDS-PAGE Klebsiella pneumoniae