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Literature summary extracted from

  • Macher, B.A.; Mudd, J.B.
    Partial purification and properties of ethanolamine kinase from spinach leaf (1976), Arch. Biochem. Biophys., 177, 24-30.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.82 EGTA stimulation at 0.4-0.9 mM, inhibition at 1.8 mM Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.82 1,10-phenanthroline
-
 Spinacia oleracea
2.7.1.82 choline not Spinacia oleracea
2.7.1.82 EDTA
-
 Spinacia oleracea
2.7.1.82 EGTA stimulation at 0.4-0.9 mM, inhibition at 1.8 mM Spinacia oleracea
2.7.1.82 MgATP2- substrate inhibition Spinacia oleracea
2.7.1.82 monomethylethanolamine
-
 Spinacia oleracea
2.7.1.82 N,N-dimethylethanolamine
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.82 0.042
-
 ethanolamine
-
 Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.82 Mg2+ Mg-ATP complex is the substrate Spinacia oleracea
2.7.1.82 Mg2+ free Mg2+ essential for maximal velocity Spinacia oleracea
2.7.1.82 Mg2+ Km MgATP2-: 0.063 mM Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.82 110000
-
 gel filtration Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.82 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.82 partial Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.82 leaf
-
 Spinacia oleracea
-

Storage Stability

EC Number Storage Stability Organism
2.7.1.82 0-4°C or -20°C, partially purified enzyme, 10-14 days, 50% loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.82 ATP + ethanolamine
-
 Spinacia oleracea ADP + O-phosphoethanolamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.82 30
-
 assay at Spinacia oleracea