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Literature summary extracted from

  • Hiles, R.A.; Henderson, LaVell M.
    The partial purification and properties of hydroxylysine kinase from rat liver (1972), J. Biol. Chem., 247, 646-651.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.81 2-Amino-5-hydroxyadipate 4 isomers, 40 mM, strong inhibition Rattus norvegicus
2.7.1.81 4,5-Dehydro-(trans)-L-lysine
-
Rattus norvegicus
2.7.1.81 carnitine 2 isomers Rattus norvegicus
2.7.1.81 choline 40 mM, weak inhibition Rattus norvegicus
2.7.1.81 D-Lysine
-
Rattus norvegicus
2.7.1.81 D-ornithine
-
Rattus norvegicus
2.7.1.81 ethanolamine 40 mM, weak inhibition Rattus norvegicus
2.7.1.81 Hydroxy-L-pipecolate
-
Rattus norvegicus
2.7.1.81 hydroxy-L-proline 40 mM, strong Rattus norvegicus
2.7.1.81 L-2,4-diamino-n-butyrate
-
Rattus norvegicus
2.7.1.81 L-homoserine 40 mM, weak inhibition Rattus norvegicus
2.7.1.81 L-lysine higher inhibitory effect than with D-lysine Rattus norvegicus
2.7.1.81 L-ornithine higher inhibitory effect than with D-ornithine Rattus norvegicus
2.7.1.81 Mn2+ at 1 mM, in presence of 1 mM Mg2+, strong inhibition, but activation in absence of Mg2+ Rattus norvegicus
2.7.1.81 S-aminoethyl-L-cysteine
-
Rattus norvegicus
2.7.1.81 Zn2+ at 1 mM, in presence of 1 mM Mg2+, strong inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.81 0.0056
-
hydroxy-L-lysine pH 8.0, 37°C Rattus norvegicus
2.7.1.81 0.0062
-
allohydroxy-L-lysine pH 8.0, 37°C Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.81 Ca2+ requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ Rattus norvegicus
2.7.1.81 Co2+ requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ Rattus norvegicus
2.7.1.81 Cu2+ requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ Rattus norvegicus
2.7.1.81 Fe2+ requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ Rattus norvegicus
2.7.1.81 Hg2+ requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+ Rattus norvegicus
2.7.1.81 Mg2+ requirement of a divalent metal ion, Mg2+ yields highest activity Rattus norvegicus
2.7.1.81 Mn2+ requirement of a divalent metal ion, 1 mM, 13% of activity compared to Mg2+, strong inhibition in presence of 1 mM Mg2+ Rattus norvegicus
2.7.1.81 additional information K+, Li+ have no influence Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.81 GTP + 5-hydroxy-L-lysine Rattus norvegicus erythro-5-hydroxy-L8-lysine GDP + O-phosphohydroxy-L-lysine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.81 Bos taurus
-
-
-
2.7.1.81 Cebus albifrons
-
-
-
2.7.1.81 Chlorocebus aethiops
-
-
-
2.7.1.81 Gallus gallus
-
-
-
2.7.1.81 Mus musculus
-
-
-
2.7.1.81 no activity in Homo sapiens
-
no activity in liver and kidney
-
2.7.1.81 Oryctolagus cuniculus
-
-
-
2.7.1.81 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.81 1200fold Rattus norvegicus
2.7.1.81 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.81 kidney
-
Mus musculus
-
2.7.1.81 kidney
-
Rattus norvegicus
-
2.7.1.81 kidney
-
Oryctolagus cuniculus
-
2.7.1.81 kidney
-
Chlorocebus aethiops
-
2.7.1.81 kidney
-
Cebus albifrons
-
2.7.1.81 liver
-
Gallus gallus
-
2.7.1.81 liver
-
Mus musculus
-
2.7.1.81 liver
-
Rattus norvegicus
-
2.7.1.81 liver
-
Bos taurus
-
2.7.1.81 liver
-
Oryctolagus cuniculus
-
2.7.1.81 liver
-
Cebus albifrons
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.81 0.114
-
-
Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.81 frozen, little loss of activity for weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.81 GTP + 5-hydroxy-L-lysine erythro-5-hydroxy-L8-lysine Rattus norvegicus GDP + O-phosphohydroxy-L-lysine
-
?
2.7.1.81 GTP + allohydroxy-L-lysine
-
Rattus norvegicus ?
-
?
2.7.1.81 additional information no activity with D-isomers, ITP can partially substitute for GTP Rattus norvegicus ?
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.81 37
-
assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.81 7.7
-
-
Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.81 6 9 25% of maximal activity at pH 6.0, 35% of maximal activity at pH 9.0 Rattus norvegicus