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Literature summary extracted from

  • Cagen, L.M.; Friedmann, H.C.
    Enzymatic phosphorylation of serine (1972), J. Biol. Chem., 247, 3382-3392.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.80 Ca2+ 50% inhibition at 1 mM Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Cd2+ 65% inhibition at 1 mM Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 D-serine 20 mM, weak inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 DL-homoserine 100 mM, weak inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 EDTA inpresence of phosphate Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 glycerol 100 mM, weak inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 glycine 20% inhibition at 10 mM Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Glycyl-L-serine weak inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Hg2+ 55% inhibition at 1 mM Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 L-alanine 35% inhibition at 10 mM Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 L-threonine 100 mM, weak inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 La3+ 25% inhibition at 1 mM Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 phosphate competitive Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Sr2+ weak inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Tris 0.6 M, weak inhibition Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Zn2+ 85% inhibition at 10 mM Propionibacterium freudenreichii subsp. shermanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.80 0.1
-
 diphosphate pH 7.8, 37°C Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 0.13
-
 Mg2+ 1 mM diphosphate, pH 7.8, 37°C Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 1.9
-
 L-serine pH 7.8, 37°C Propionibacterium freudenreichii subsp. shermanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.80 Ba2+ slight stimulation, about 14% of activity compared to Mg2+ Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Co2+ slight stimulation, about 42% of activity compared to Mg2+ Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Cu2+ slight stimulation, about 14% of activity compared to Mg2+ Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Mg2+ high stimulation Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Mn2+ slight stimulation, about 34% of activity compared to Mg2+ Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 Zn2+ slight stimulation, about 14% of activity compared to Mg2+ Propionibacterium freudenreichii subsp. shermanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.80 65000
-
 gel filtration Propionibacterium freudenreichii subsp. shermanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.80 diphosphate + L-serine Propionibacterium freudenreichii subsp. shermanii
-
 phosphate + O-phospho-L-serine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.80 Propionibacterium freudenreichii subsp. shermanii
-
 strain ATCC 9614
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.80 partial, 102fold Propionibacterium freudenreichii subsp. shermanii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.80 diphosphate + L-serine = phosphate + O-phospho-L-serine binary or ping-pong mechanism Propionibacterium freudenreichii subsp. shermanii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.80 9.5
-
 partially purified enzyme Propionibacterium freudenreichii subsp. shermanii

Storage Stability

EC Number Storage Stability Organism
2.7.1.80 4°C o r-15°C, 0.02 M Tris buffer, pH 7.8, stable for several weeks Propionibacterium freudenreichii subsp. shermanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.80 diphosphate + alpha-methylserine 7% activity compared to L-serine Propionibacterium freudenreichii subsp. shermanii phosphate + alpha-methyl-O-phospho-L-serine
-
 ?
2.7.1.80 diphosphate + DL-homoserine 7% activity compared to L-serine Propionibacterium freudenreichii subsp. shermanii phosphate + O-phospho-DL-homoserine
-
 ?
2.7.1.80 diphosphate + glycyl-L-serine 16% activity compared to L-serine Propionibacterium freudenreichii subsp. shermanii phosphate + glycyl-O-phospho-L-serine
-
 ?
2.7.1.80 diphosphate + L-serine
-
 Propionibacterium freudenreichii subsp. shermanii phosphate + O-phospho-L-serine
-
 ?
2.7.1.80 diphosphate + L-threonine 8% activity compared to L-serine Propionibacterium freudenreichii subsp. shermanii phosphate + O-phospho-L-threonine
-
 ?
2.7.1.80 diphosphate + N-chloroacetyl-L-serine 19% activity compared to L-serine Propionibacterium freudenreichii subsp. shermanii phosphate + N-chloroacetyl-O-phospho-L-serine
-
 ?
2.7.1.80 additional information substrate specificity Propionibacterium freudenreichii subsp. shermanii ?
-
 ?
2.7.1.80 additional information no activity with D-serine and L-cysteine, tripolyphosphate can substitute to some extent for diphosphate Propionibacterium freudenreichii subsp. shermanii ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.80 37
-
 assay at Propionibacterium freudenreichii subsp. shermanii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.80 30
-
 stable at least 12 h Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 37
-
 stable at least 1 h Propionibacterium freudenreichii subsp. shermanii
2.7.1.80 65
-
 15 min, 46% remaining activity Propionibacterium freudenreichii subsp. shermanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.80 7
-
-
 Propionibacterium freudenreichii subsp. shermanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.80 5.4 7.8 60% of maximal activity at pH 5.4 and pH 7.8 Propionibacterium freudenreichii subsp. shermanii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.80 0.8
-
 glycine pH 7.8, 37°C Propionibacterium freudenreichii subsp. shermanii