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Literature summary extracted from

  • Park, J.K.; Wang, L.X.; Roseman, S.
    Isolation of a glucosamine-specific kinase, a unique enzyme of Vibrio cholerae (2002), J. Biol. Chem., 277, 15573-15578.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.8
-
 Vibrio cholerae serotype O1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.8 0.48
-
 D-glucosamine pH 7.0, 25°C Vibrio cholerae serotype O1
2.7.1.8 1.96
-
 ATP pH 7.0, 25°C Vibrio cholerae serotype O1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.8 Mg2+ Km: 1.98 mM Vibrio cholerae serotype O1

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.8 30000
-
 x * 30000, SDS-PAGE Vibrio cholerae serotype O1
2.7.1.8 31600
-
 x * 31600, calculation from nucleotide sequence Vibrio cholerae serotype O1

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.8 Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.8 recombinant enzyme Vibrio cholerae serotype O1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.8 92.5
-
-
 Vibrio cholerae serotype O1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.8 ATP + D-glucosamine enzyme is specific for D-glucosamine Vibrio cholerae serotype O1 ADP + D-glucosamine 6-phosphate
-
 ?
2.7.1.8 GTP + D-glucosamine 10% of the activity with ATP Vibrio cholerae serotype O1 GDP + D-glucosamine 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.8 ? x * 30000, SDS-PAGE Vibrio cholerae serotype O1
2.7.1.8 ? x * 31600, calculation from nucleotide sequence Vibrio cholerae serotype O1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.8 40 42
-
 Vibrio cholerae serotype O1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.8 4 30 30 min, stable Vibrio cholerae serotype O1
2.7.1.8 37
-
 30 min, retains at least 91% of its activity Vibrio cholerae serotype O1
2.7.1.8 65
-
 30 min, complete loss of activity Vibrio cholerae serotype O1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.8 7.5 8.5
-
 Vibrio cholerae serotype O1