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Literature summary extracted from

  • Wang, L.K.; Shuman, S.
    Mutational analysis defines the 5'-kinase and 3'-phosphatase active sites of T4 polynucleotide kinase (2002), Nucleic Acids Res., 30, 1073-1080.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.78 D165A site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining Tequatrovirus T4
2.7.1.78 D180A site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity Tequatrovirus T4
2.7.1.78 D250A site-directed mutagenesis, increased activity compared to the wild-type , reduced 3'-phosphatase activity Tequatrovirus T4
2.7.1.78 D254A site-directed mutagenesis, increased activity compared to the wild-type, nearly no 3'-phosphatase activity remaining Tequatrovirus T4
2.7.1.78 D278A site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining Tequatrovirus T4
2.7.1.78 K125A site-directed mutagenesis, slightly higher activity than the wild-type Tequatrovirus T4
2.7.1.78 R122A site-directed mutagenesis, slightly higher activity than the wild-type, reduced 3'-phosphatase activity Tequatrovirus T4
2.7.1.78 R126A site-directed mutagenesis, highly reduced activity Tequatrovirus T4
2.7.1.78 R176A site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity Tequatrovirus T4
2.7.1.78 R213A site-directed mutagenesis, increased activity compared to the wild-type, no 3'-phosphatase activity remaining Tequatrovirus T4
2.7.1.78 R246A site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity Tequatrovirus T4
2.7.1.78 R279A site-directed mutagenesis, increased activity compared to the wild-type, highly reduced 3'-phosphatase activity Tequatrovirus T4
2.7.1.78 R36A site-directed mutagenesis, similar activity than the wild-type Tequatrovirus T4
2.7.1.78 R38A site-directed mutagenesis, highly reduced activity, reduced 3'-phosphatase activity Tequatrovirus T4
2.7.1.78 S84A site-directed mutagenesis, slightly lower activity than the wild-type, reduced 3'-phosphatase activity Tequatrovirus T4
3.1.3.32 D165A inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D165E inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D165N inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D167A inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D167E inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D167N inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D176A inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D176K inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D213A inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D213K inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D254A inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D254E inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D254N inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D278A inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 D278E partial inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 R126A inactivation of 5’-kinase activity Tequatrovirus T4
3.1.3.32 R126K inactivation of 5’-kinase activity Tequatrovirus T4
3.1.3.32 R126Q inactivation of 5’-kinase activity Tequatrovirus T4
3.1.3.32 R246A partial inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 R246K no inactivation Tequatrovirus T4
3.1.3.32 R279A partial inactivation of 3’-phosphatase activity Tequatrovirus T4
3.1.3.32 R38A inactivation of 5’-kinase activity Tequatrovirus T4
3.1.3.32 R38K inactivation of 5’-kinase activity Tequatrovirus T4
3.1.3.32 R38Q inactivation of 5’-kinase activity Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 diphosphate
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-RNA Tequatrovirus T4 involved in repair of broken RNA termini ADP + 5'-phospho-RNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Tequatrovirus T4
-
 from infected Escherichia coli
-
3.1.3.32 Tequatrovirus T4
-
 bifunctional 5'-kinase/3'-phosphatase
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.1.78 ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA 5 amino acids, Lys15, Ser116, Asp35, Arg38, and Arg126, comprise the 5'-kinase active site, structure-activity relationships Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-RNA
-
 Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA involved in repair of broken RNA termini Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide
-
 Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Tequatrovirus T4 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Tequatrovirus T4