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Literature summary extracted from

  • Bosdal, T.; Lillehaug, J.R.
    Purification and kinetic properties of polynucleotide kinase from rat testes (1985), Biochim. Biophys. Acta, 840, 280-286.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.78 spermine 1 mM enhances activity 3times Rattus norvegicus
2.7.1.78 spermine inhibition above 1 mM Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 diphosphate
-
 Rattus norvegicus
2.7.1.78 phosphate
-
 Rattus norvegicus
2.7.1.78 spermine 1 mM enhances activity 3times, inhibition above 1 mM Rattus norvegicus
2.7.1.78 sulfate
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 divalent cations absolutely required Rattus norvegicus
2.7.1.78 K+ stimulates, optimal concentration: 0.1 M Rattus norvegicus
2.7.1.78 Mg2+
-
 Rattus norvegicus
2.7.1.78 Mn2+ can partially replace Mg2+ in activation Rattus norvegicus
2.7.1.78 Na+ stimulates, optimal concentration: 0.1 M Rattus norvegicus
2.7.1.78 NH4+ stimulates, optimal concentration 0.1 M Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 38000
-
 gel filtration Rattus norvegicus
2.7.1.78 40000
-
 1 * 40000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Rattus norvegicus
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 over 1875fold, to homogeneity Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 0.005
-
 above, purified enzyme, 30°C Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.7.1.78 -20°C, 10 mM Na2HPO4, pH 7.4, 50% v/v glycerol, 1 mM DTT, 0.001 mM ATP, stable for 2 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA nuclease-treated calf thymus DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA 5'-HO-tRNA is a very poor substrate Rattus norvegicus ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide (dT)10 Rattus norvegicus ADP + oligonucleotide 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 monomer 1 * 40000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 5.3
-
-
 Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Rattus norvegicus