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Literature summary extracted from

  • Sertic-Pritsos, K.; Vinocour, M.; Winicov, I.
    5-Hydroxyl RNA kinase from mouse L cells (1984), Eur. J. Biochem., 144, 47-55.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 Mn2+ inhibition above 1 mM Mus musculus
2.7.1.78 NH4+ weak inhibition Mus musculus
2.7.1.78 sulfate weak Mus musculus
2.7.1.78 Zn2+ inhibition in presence of Mg2+ Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 0.0036
-
 5'-hydroxyl poly(A) pH 8.4, 37°C Mus musculus
2.7.1.78 0.22
-
 [gamma-S]ATP pH 8.4, 37°C Mus musculus
2.7.1.78 0.53
-
 [gamma-S]GTP pH 8.4, 37°C Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Ca2+ about 17% of the activity with Mg2+, synergistic to Mg2+ Mus musculus
2.7.1.78 Mg2+ required Mus musculus
2.7.1.78 Mn2+ no activation, 50% inhibition at 1 mM Mus musculus
2.7.1.78 NH4+ slightly inhibitory Mus musculus
2.7.1.78 Zn2+ no activation, inhibition in presence of Mg2+ Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 93000
-
 1 * 93000, SDS-PAGE Mus musculus
2.7.1.78 96000
-
 gel filtration, native PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 partial, 35fold Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 cell suspension culture L-cells Mus musculus
-
2.7.1.78 L-cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 0.0007
-
 partially purified enzyme, substrate RNA Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA low activity Mus musculus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA micrococcal-nuclease-treated calf thymus DNA is not effectively phosphorylated Mus musculus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA much more efficient as substrate than DNA Mus musculus ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + 5'-hydroxyl poly(A)
-
 Mus musculus ?
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide 5'-hydroxyl poly(A) Mus musculus ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 [gamma-S]ATP + 5'-dephospho-RNA
-
 Mus musculus [gamma-S]ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 [gamma-S]GTP + 5'-dephospho-RNA
-
 Mus musculus [gamma-S]GDP + 5'-phospho-RNA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 monomer 1 * 93000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 5.5
-
 substrate DNA Mus musculus
2.7.1.78 9
-
 substrate RNA Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.78 7.5 9.4 pH 7.5: about 50% of activity maximum, pH 8.4 and pH 9.4: about 85% of activity maximum Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 4-(3-bromo-8-chloro-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-yl)-N1-cyclohexyl-N2-[2-(1H-imidazol-4-yl)ethyl]piperazine-1,2-dicarboxamide
-
 Mus musculus
2.7.1.78 4-(3-bromo-8-chloro-6,11-dihydro-5H-benzo[5,6]cyclohepta[1,2-b]pyridin-11-yl)-N1-cyclohexyl-N2-[3-(1H-imidazol-4-yl)propyl]piperazine-1,2-dicarboxamide
-
 Mus musculus
2.7.1.78 ATP
-
 Mus musculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.1.78 Mus musculus
-
-
 4.8