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Literature summary extracted from

  • Lillehaug, J.R.
    Inhibition of T4 polynucleotide kinase by the ATP analog, beta, gamma-imidoadenylyl 5-triphosphate (1978), Biochim. Biophys. Acta, 525, 357-363.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.7.1.78 synthesis production of beta,gamma-imidoadenylyl 5'-tetraphosphate by using beta,gamma-imidoadenylyl 5'-triphosphate as substrate in the reverse reaction Tequatrovirus T4

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 beta,gamma-imidoadenylyl 5'-tetraphosphate ATP analog, competitive against ATP, noncompetitive against 5'-OH-DNA, serves as substrate for the reverse reaction only Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Mg2+
-
 Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 r
2.7.1.78 ATP + synthetic oligonucleotide oligo dpT(pT)9 Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide oligodeoxynucleotides of chain length less than approximately 10-12 residues Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
 ?
2.7.1.78 beta,gamma-imidoadenylyl 5'-triphosphate + 5'-phospho-DNA ATP analog, inhibitory, competitive against ATP Tequatrovirus T4 beta,gamma-imidoadenylyl 5'-tetraphosphate + 5'-dephospho-DNA
-
 ir
2.7.1.78 beta,gamma-imidoadenylyl 5'-triphosphate + 5'-phospho-DNA is no substrate in the forward reaction but can replace ADP and ATP in the reverse reaction Tequatrovirus T4 beta,gamma-imidoadenylyl 5'-tetraphosphate + 5'-dephospho-DNA
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 35
-
 assay at Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 8
-
 assay at Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Tequatrovirus T4

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.78 3
-
 beta,gamma-imidoadenylyl 5'-tetraphosphate versus ATP, pH 8.0, 35°C, independent of pH-value Tequatrovirus T4
2.7.1.78 11
-
 beta,gamma-imidoadenylyl 5'-tetraphosphate versus 5'-OH-DNA, pH 8.0, 35°C, independent of pH-value Tequatrovirus T4