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Literature summary extracted from

  • Tamura, S.; Teraoka, H.; Tsukada, K.
    Characterization of DNA kinase from calf thymus (1981), Eur. J. Biochem., 115, 449-453.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.78 polyamines stimulate at suboptimal Mg2+ concentration Bos taurus
2.7.1.78 spermidine 3-4fold activation, activates by lowering the Km for Mg2+ and enhancing Vmax, optimal at 10-15 mM Bos taurus
2.7.1.78 spermine activates 3-4fold at 2 mM Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 ammonium sulfate about 75% inhibition at 10 mM Bos taurus
2.7.1.78 diphosphate weak Bos taurus
2.7.1.78 p-chloromercuribenzoate reversible Bos taurus
2.7.1.78 sulfate weak Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 0.004
-
 ATP pH 5.5, 37°C Bos taurus
2.7.1.78 2.5
-
 Mg2+ pH 5.5, 37°C Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 K+ KCl slightly stimulates Bos taurus
2.7.1.78 Mg2+ required Bos taurus
2.7.1.78 Mg2+ optimal concentration is 10 mM Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 54000
-
 1 * 54000, SDS-PAGE Bos taurus
2.7.1.78 56000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Bos taurus
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Bos taurus
-
 calf
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78 to homogeneity Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 0.0167
-
 purified enzyme Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.7.1.78 0°C, stable for at least 1 month Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Bos taurus ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA no activity Bos taurus ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + synthetic oligonucleotide no activity with poly(A) Bos taurus ADP + oligonucleotide 5'-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 monomer 1 * 54000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 25 30
-
 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 5.5
-
 sharp maximum Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.78 5
-
 diphosphate pH 5.5, 25°C Bos taurus
2.7.1.78 7
-
 sulfate pH 5.5, 25°C Bos taurus