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Literature summary extracted from

  • Novogrodsky, A.; Tal, M.; Traub, A.; Hurwitz, J.
    The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5-hydroxyl polynucleotide kinase (1966), J. Biol. Chem., 241, 2933-2943.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.78 greatest protection from heat inactivation with substrates for the kinase, e.g. thymus DNA and ribosomal RNA, both micrococcal nuclease-treated and 3'-AMP Escherichia phage T2

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 Ca2+ inhibits in combination with MgCl2, stimulates without MgCl2 Escherichia phage T2
2.7.1.78 Cu2+ inhibits in presence of CuCl2 Escherichia phage T2
2.7.1.78 diphosphate
-
 Escherichia phage T2
2.7.1.78 Maleate
-
 Escherichia phage T2
2.7.1.78 Ni2+ inhibits in combination with MgCl2, stimulates without MgCl2 Escherichia phage T2
2.7.1.78 phosphate
-
 Escherichia phage T2

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 0.1
-
 Mn2+ pH 6.0, 37°C Escherichia phage T2
2.7.1.78 0.5
-
 Mg2+ pH 6.0, 37°C Escherichia phage T2

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Ca2+ divalent cation required, slight activation in absence of MgCl2 Escherichia phage T2
2.7.1.78 Ca2+ inhibition in combination with MgCl2 Escherichia phage T2
2.7.1.78 Co2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective in activation Escherichia phage T2
2.7.1.78 divalent cations absolutely required Escherichia phage T2
2.7.1.78 divalent cations Mg2+, Mn2+, Zn2+, Co2+ are equally effective Escherichia phage T2
2.7.1.78 Mg2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective Escherichia phage T2
2.7.1.78 Mn2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective Escherichia phage T2
2.7.1.78 Ni2+ slight activation in absence of MgCl2, inhibition in combination with MgCl2 Escherichia phage T2
2.7.1.78 Zn2+ Mg2+, Mn2+, Zn2+ and Co2+ are equally effective in activation Escherichia phage T2

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Escherichia phage T2
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Escherichia phage T2
-
 from infected Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 2'(3')-ribonucleotides no activity with (2')-AMP Escherichia phage T2 ?
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Escherichia phage T2 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA
-
 Escherichia phage T2 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + nucleoside-3'-monophosphate
-
 Escherichia phage T2 ADP + nucleoside-3',5'-diphosphate
-
 ?
2.7.1.78 CTP + 5'-dephospho-DNA
-
 Escherichia phage T2 CDP + 5'-phospho-DNA
-
 ?
2.7.1.78 GTP + 5'-dephospho-DNA
-
 Escherichia phage T2 GDP + 5'-phospho-DNA
-
 ?
2.7.1.78 additional information substrate specificity Escherichia phage T2 ?
-
 ?
2.7.1.78 additional information no activity with adenosine, nucleotides, and 5'-mononucleotides Escherichia phage T2 ?
-
 ?
2.7.1.78 UTP + 5'-dephospho-DNA
-
 Escherichia phage T2 UDP + 5'-phospho-DNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 37
-
 assay at Escherichia phage T2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.78 additional information
-
 protection against heat inactivation by substrates, e.g. calf thymus DNA and ribosomal RNA, both micrococcal nuclease-treated, and 3'-AMP Escherichia phage T2
2.7.1.78 37
-
 activity is completely lost without substrates Escherichia phage T2

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 6 9 broad optimum Escherichia phage T2

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.78 5 9.5 about 50% of activity maximum at pH 5.0 and pH 9.5 Escherichia phage T2

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Escherichia phage T2
2.7.1.78 CDP
-
 Escherichia phage T2
2.7.1.78 CTP
-
 Escherichia phage T2
2.7.1.78 GDP
-
 Escherichia phage T2
2.7.1.78 GTP
-
 Escherichia phage T2
2.7.1.78 UDP
-
 Escherichia phage T2
2.7.1.78 UTP
-
 Escherichia phage T2