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Literature summary extracted from

  • Novogrodsky, A.; Hurwitz, J.
    The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5-hydroxyl termini (1966), J. Biol. Chem., 241, 2923-2932.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.1.78 freezing and thawing reduces activity after 1 cycle by 60% Tequatrovirus T4
2.7.1.78 freezing and thawing reduces activity after 1 cycle by 60% Escherichia phage T2

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 chloramphenicol inhibits the formation of enzyme in cells cotransfected with bacteriophage and chloramphenicol Escherichia phage T2
2.7.1.78 chloramphenicol inhibits the formation of enzyme in cells cotransfected with bacteriophage and chloramphenicol Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 Mg2+
-
 Escherichia phage T2
2.7.1.78 Mg2+ required Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Tequatrovirus T4
-
 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA Escherichia phage T2
-
 ADP + 5'-phospho-DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Escherichia phage T2
-
 from infected Escherichia coli
-
2.7.1.78 no activity in bacteriophage T1
-
-
-
2.7.1.78 no activity in bacteriophage T5
-
-
-
2.7.1.78 Tequatrovirus T4
-
 from infected Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.78
-
 Escherichia phage T2

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 0.53
-
 purified enzyme Tequatrovirus T4

Storage Stability

EC Number Storage Stability Organism
2.7.1.78 0°C, partially purified ammonium sulfate fraction, stable for more than 1 year Escherichia phage T2
2.7.1.78 0°C, purified protein, concentration 0.014 mg/ml, stable for 5 months Escherichia phage T2

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 2'(3')-ribonucleotides no activity with 2'(3')-AMP and 2'(3')-CMP Escherichia phage T2 ?
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA
-
 Escherichia phage T2 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA micrococcal-nuclease-treated calf thymus DNA Tequatrovirus T4 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-DNA micrococcal-nuclease-treated calf thymus DNA Escherichia phage T2 ADP + 5'-phospho-DNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA
-
 Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA soluble and ribosomal RNA of Escherichia coli Escherichia phage T2 ADP + 5'-phospho-RNA
-
 ?
2.7.1.78 ATP + 5'-dephospho-RNA synthetic polynucleotides Escherichia phage T2 ADP + 5'-phospho-RNA
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 38
-
 assay at Tequatrovirus T4
2.7.1.78 38
-
 assay at Escherichia phage T2

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.78 25
-
 marked temperature lability in absence of substrate Tequatrovirus T4
2.7.1.78 25
-
 marked temperature lability in absence of substrate Escherichia phage T2
2.7.1.78 37
-
-
 Escherichia phage T2
2.7.1.78 37
-
 15 min, 77% loss of activity Tequatrovirus T4
2.7.1.78 45
-
 5 min, loss of 94% activity with substrate DNA and 91% activity with substrate RNA Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 6
-
 assay at Tequatrovirus T4
2.7.1.78 6
-
 assay at Escherichia phage T2

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ATP dependent on Tequatrovirus T4
2.7.1.78 ATP dependent on Escherichia phage T2