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Literature summary extracted from

  • Maunders, M.J.
    Polynucleotide kinase (EC 2.7.1.78) (1993), Methods Mol. Biol., 16, 343-357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.78 polyamines stimulate Homo sapiens
2.7.1.78 polyamines stimulate Rattus norvegicus
2.7.1.78 polyamines stimulate Bos taurus
2.7.1.78 polyamines stimulate Mesocricetus auratus
2.7.1.78 polyamines stimulate Tequatrovirus T4
2.7.1.78 polyamines stimulate Escherichia phage T2
2.7.1.78 polyamines stimulate Enterobacteria phage T6
2.7.1.78 Polyethylene glycol 4-10%, stimulates Tequatrovirus T4
2.7.1.78 spermidine
-
Rattus norvegicus
2.7.1.78 spermidine
-
Bos taurus
2.7.1.78 spermidine stimulates Tequatrovirus T4
2.7.1.78 sulfhydryl reagents
-
Homo sapiens
2.7.1.78 sulfhydryl reagents essential for activity, maximal activity with 5 mM DTT, less stimulation with 2-mercaptoethanol and glutathione Tequatrovirus T4

Application

EC Number Application Comment Organism
2.7.1.78 synthesis due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates Rattus norvegicus
2.7.1.78 synthesis due to reversibility of the reaction, the bacteriophage can be utilized for exchange of labeled phosphate groups between 2 substrates Tequatrovirus T4

General Stability

EC Number General Stability Organism
2.7.1.78 freezing and thawing reduces activity after 1 cycle by 60% Tequatrovirus T4
2.7.1.78 freezing and thawing reduces activity after 1 cycle by 60% Escherichia phage T2

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.78 diphosphate
-
Bos taurus
2.7.1.78 diphosphate
-
Escherichia phage T2
2.7.1.78 diphosphate
-
Homo sapiens
2.7.1.78 diphosphate
-
Rattus norvegicus
2.7.1.78 diphosphate
-
Tequatrovirus T4
2.7.1.78 KCl
-
Bos taurus
2.7.1.78 KCl
-
Homo sapiens
2.7.1.78 KCl KCl stimulates at low concentrations, inhibits at high concentrations Tequatrovirus T4
2.7.1.78 NaCl
-
Bos taurus
2.7.1.78 NaCl
-
Homo sapiens
2.7.1.78 NaCl
-
Rattus norvegicus
2.7.1.78 NaCl stimulates activity towards single stranded substrates, inhibitory with some duplexes Tequatrovirus T4
2.7.1.78 NH4+
-
Homo sapiens
2.7.1.78 NH4+
-
Tequatrovirus T4
2.7.1.78 phosphate
-
Bos taurus
2.7.1.78 phosphate
-
Escherichia phage T2
2.7.1.78 phosphate
-
Homo sapiens
2.7.1.78 phosphate
-
Rattus norvegicus
2.7.1.78 phosphate
-
Tequatrovirus T4

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.78 additional information
-
additional information
-
Tequatrovirus T4
2.7.1.78 0.004
-
ATP
-
Bos taurus
2.7.1.78 0.004
-
ATP phosphate exchange reaction between nucleotides, forward reaction, pH 7.6, 37°C Tequatrovirus T4
2.7.1.78 0.0076
-
5'-OH-DNA large DNA fragments released by nuclease treatment, pH 7.6, 37°C Tequatrovirus T4
2.7.1.78 0.013 0.14 ATP depending on DNA acceptor, pH 7.6, 37°C Tequatrovirus T4
2.7.1.78 0.014
-
ATP
-
Escherichia phage T2
2.7.1.78 0.015
-
UTP
-
Escherichia phage T2
2.7.1.78 0.0222 0.143 oligonucleotides depending on the 5'-base and the length of the oligonucleotide, pH 7.6, 37°C Tequatrovirus T4
2.7.1.78 0.025
-
CTP
-
Escherichia phage T2
2.7.1.78 0.033
-
GTP
-
Escherichia phage T2
2.7.1.78 0.2
-
ADP dephosphorylation of single-stranded oligonucleotides, pH 7.6, 37°C Tequatrovirus T4
2.7.1.78 0.2
-
ADP phosphate exchange reaction between nucleotides, reverse reaction, pH 7.6, 37°C Tequatrovirus T4

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.78 K+
-
Rattus norvegicus
2.7.1.78 K+
-
Bos taurus
2.7.1.78 K+ KCl stimulates at low concentrations, inhibits at high concentrations Tequatrovirus T4
2.7.1.78 Mg2+
-
Homo sapiens
2.7.1.78 Mg2+
-
Rattus norvegicus
2.7.1.78 Mg2+
-
Escherichia phage T2
2.7.1.78 Mg2+ required Bos taurus
2.7.1.78 Mg2+ required Tequatrovirus T4
2.7.1.78 Mg2+ optimal concentration is 10 mM Bos taurus
2.7.1.78 Mg2+ optimal concentration is 10 mM Tequatrovirus T4
2.7.1.78 Mg2+ optimal concentration at pH optimum 7.6 is 10 mM Tequatrovirus T4
2.7.1.78 Mn2+
-
Bos taurus
2.7.1.78 Mn2+
-
Escherichia phage T2
2.7.1.78 Mn2+ can partially replace Mg2+ in activation Homo sapiens
2.7.1.78 Mn2+ can partially replace Mg2+ in activation Rattus norvegicus
2.7.1.78 Mn2+ can partially replace Mg2+ in activation Tequatrovirus T4
2.7.1.78 Mn2+ at 3.3 mM 50% of the activity with 10 mM Mg2+ Tequatrovirus T4
2.7.1.78 Na+
-
Rattus norvegicus
2.7.1.78 Na+ stimulates activity towards single stranded substrates, inhibitory to some duplexes Tequatrovirus T4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.78 33000
-
4 * 33000, SDS-PAGE Tequatrovirus T4
2.7.1.78 33200
-
4 * 33200, analytical ultracentrifugation Tequatrovirus T4
2.7.1.78 80000
-
gel filtration Rattus norvegicus
2.7.1.78 140000
-
gel filtration Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA Homo sapiens
-
ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA Rattus norvegicus
-
ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA Rattus norvegicus
-
ADP + 5'-phospho-DNA
-
r
2.7.1.78 ATP + 5'-dephospho-DNA Bos taurus
-
ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA Tequatrovirus T4
-
ADP + 5'-phospho-DNA
-
r
2.7.1.78 ATP + 5'-dephospho-DNA Escherichia phage T2
-
ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA Enterobacteria phage T6
-
ADP + 5'-phospho-DNA
-
?
2.7.1.78 additional information Tequatrovirus T4 in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.78 Bos taurus
-
calf
-
2.7.1.78 Enterobacteria phage T6
-
-
-
2.7.1.78 Escherichia phage T2
-
from infected Escherichia coli
-
2.7.1.78 Homo sapiens
-
HeLa cells
-
2.7.1.78 Mesocricetus auratus
-
chinese hamster lung cells
-
2.7.1.78 no activity in Escherichia coli
-
-
-
2.7.1.78 Rattus norvegicus
-
-
-
2.7.1.78 Tequatrovirus T4
-
from infected Escherichia coli
-
2.7.1.78 Tequatrovirus T4
-
gene pseT
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.78 cell culture HeLa cells Homo sapiens
-
2.7.1.78 cell culture chinese hamster lung cells Mesocricetus auratus
-
2.7.1.78 HeLa cell
-
Homo sapiens
-
2.7.1.78 liver
-
Homo sapiens
-
2.7.1.78 liver
-
Rattus norvegicus
-
2.7.1.78 liver
-
Bos taurus
-
2.7.1.78 liver
-
Mesocricetus auratus
-
2.7.1.78 lung
-
Mesocricetus auratus
-
2.7.1.78 thymus
-
Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.78 30 40 commercial preparation Tequatrovirus T4

Storage Stability

EC Number Storage Stability Organism
2.7.1.78 -20°C, 50 mM Tris-HCl, pH 7.5, 25 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.001 mM ATP, 50% glycerol, stable for up to 18 months Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.78 ATP + 5'-dephospho-DNA
-
Homo sapiens ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA
-
Rattus norvegicus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA
-
Rattus norvegicus ADP + 5'-phospho-DNA
-
r
2.7.1.78 ATP + 5'-dephospho-DNA
-
Bos taurus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA
-
Mesocricetus auratus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA
-
Tequatrovirus T4 ADP + 5'-phospho-DNA
-
r
2.7.1.78 ATP + 5'-dephospho-DNA
-
Escherichia phage T2 ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA
-
Enterobacteria phage T6 ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA double stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA double stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
r
2.7.1.78 ATP + 5'-dephospho-DNA double stranded DNA Bos taurus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA double stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored ?
2.7.1.78 ATP + 5'-dephospho-DNA double stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored r
2.7.1.78 ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Rattus norvegicus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Rattus norvegicus ADP + 5'-phospho-DNA
-
r
2.7.1.78 ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored ?
2.7.1.78 ATP + 5'-dephospho-DNA large DNA fragments released by nuclease treatment Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored r
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Rattus norvegicus ADP + 5'-phospho-DNA
-
r
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Bos taurus ADP + 5'-phospho-DNA
-
?
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored ?
2.7.1.78 ATP + 5'-dephospho-DNA single stranded DNA Tequatrovirus T4 ADP + 5'-phospho-DNA excess ADP will cause the reverse reaction to be favored r
2.7.1.78 ATP + 5'-dephospho-RNA
-
Tequatrovirus T4 ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA
-
Escherichia phage T2 ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA
-
Enterobacteria phage T6 ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA no activity Homo sapiens ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA no activity Rattus norvegicus ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA no activity Bos taurus ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA no activity Mesocricetus auratus ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA low activity Bos taurus ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA enzyme cannot act on RNA less than 10 bases in length Rattus norvegicus ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + 5'-dephospho-RNA solely RNA-specific Rattus norvegicus ADP + 5'-phospho-RNA
-
?
2.7.1.78 ATP + deoxynucleoside 3'-phosphate
-
Tequatrovirus T4 ADP + deoxynucleoside 3',5'-diphosphate
-
?
2.7.1.78 ATP + nucleoside-3'-monophosphate
-
Rattus norvegicus ADP + nucleoside-3',5'-diphosphate
-
?
2.7.1.78 ATP + nucleoside-3'-monophosphate
-
Tequatrovirus T4 ADP + nucleoside-3',5'-diphosphate
-
?
2.7.1.78 ATP + nucleoside-3'-monophosphate
-
Escherichia phage T2 ADP + nucleoside-3',5'-diphosphate
-
?
2.7.1.78 ATP + oligonucleotides
-
Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
?
2.7.1.78 ATP + synthetic oligonucleotide
-
Homo sapiens ADP + oligonucleotide 5'-phosphate
-
?
2.7.1.78 ATP + synthetic oligonucleotide
-
Bos taurus ADP + oligonucleotide 5'-phosphate
-
?
2.7.1.78 ATP + synthetic oligonucleotide
-
Tequatrovirus T4 ADP + oligonucleotide 5'-phosphate
-
?
2.7.1.78 ATP + synthetic oligonucleotide oligodeoxynucleotides of chain length above 10-12 residues Rattus norvegicus ADP + oligonucleotide 5'-phosphate
-
?
2.7.1.78 CTP + 5'-dephospho-DNA
-
Homo sapiens CDP + 5'-phospho-DNA
-
?
2.7.1.78 CTP + 5'-dephospho-DNA
-
Rattus norvegicus CDP + 5'-phospho-DNA
-
?
2.7.1.78 CTP + 5'-dephospho-DNA
-
Bos taurus CDP + 5'-phospho-DNA
-
?
2.7.1.78 CTP + 5'-dephospho-DNA
-
Mesocricetus auratus CDP + 5'-phospho-DNA
-
?
2.7.1.78 CTP + 5'-dephospho-DNA
-
Escherichia phage T2 CDP + 5'-phospho-DNA
-
?
2.7.1.78 dATP + 5'-dephospho-DNA
-
Tequatrovirus T4 dADP + 5'-phospho-DNA
-
?
2.7.1.78 GTP + 5'-dephospho-DNA
-
Homo sapiens GDP + 5'-phospho-DNA
-
?
2.7.1.78 GTP + 5'-dephospho-DNA
-
Rattus norvegicus GDP + 5'-phospho-DNA
-
?
2.7.1.78 GTP + 5'-dephospho-DNA
-
Bos taurus GDP + 5'-phospho-DNA
-
?
2.7.1.78 GTP + 5'-dephospho-DNA
-
Mesocricetus auratus GDP + 5'-phospho-DNA
-
?
2.7.1.78 GTP + 5'-dephospho-DNA
-
Escherichia phage T2 GDP + 5'-phospho-DNA
-
?
2.7.1.78 additional information
-
Escherichia phage T2 ?
-
?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Homo sapiens ?
-
?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Rattus norvegicus ?
-
?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Bos taurus ?
-
?
2.7.1.78 additional information enzyme also has DNA 3'-phosphatase activity Tequatrovirus T4 ?
-
?
2.7.1.78 additional information in vivo role is possibly in maintaining DNA or RNA in the 5'-phosphorylated 3'-hydroxylated state which is the substrate for many reactions such as ligation and packaging Tequatrovirus T4 ?
-
?
2.7.1.78 TTP + 5'-dephospho-DNA
-
Tequatrovirus T4 TDP + 5'-phospho-DNA
-
?
2.7.1.78 UTP + 5'-dephospho-DNA
-
Rattus norvegicus UDP + 5'-phospho-DNA
-
?
2.7.1.78 UTP + 5'-dephospho-DNA
-
Tequatrovirus T4 UDP + 5'-phospho-DNA
-
?
2.7.1.78 UTP + 5'-dephospho-DNA
-
Escherichia phage T2 UDP + 5'-phospho-DNA
-
?

Subunits

EC Number Subunits Comment Organism
2.7.1.78 tetramer 4 * 33000, SDS-PAGE Tequatrovirus T4
2.7.1.78 tetramer 4 * 33200, analytical ultracentrifugation Tequatrovirus T4

Synonyms

EC Number Synonyms Comment Organism
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Homo sapiens
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Rattus norvegicus
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Bos taurus
2.7.1.78 More enzyme also possesses a 3'-phosphatase activity Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.78 37
-
-
Homo sapiens
2.7.1.78 37
-
-
Rattus norvegicus
2.7.1.78 37
-
-
Bos taurus
2.7.1.78 37
-
-
Tequatrovirus T4
2.7.1.78 37
-
-
Escherichia phage T2

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.78
-
37 0°C: about 7% of activity maximum, 37°C: activity maximum, bacteriophage T4 enzyme Tequatrovirus T4

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.78 25
-
marked temperature lability in absence of substrate Homo sapiens
2.7.1.78 25
-
marked temperature lability in absence of substrate Rattus norvegicus
2.7.1.78 25
-
marked temperature lability in absence of substrate Bos taurus
2.7.1.78 25
-
marked temperature lability in absence of substrate Mesocricetus auratus
2.7.1.78 25
-
marked temperature lability in absence of substrate Tequatrovirus T4
2.7.1.78 25
-
marked temperature lability in absence of substrate Escherichia phage T2
2.7.1.78 25
-
marked temperature lability in absence of substrate Enterobacteria phage T6
2.7.1.78 37
-
-
Escherichia phage T2
2.7.1.78 37
-
15 min, 77% loss of activity Tequatrovirus T4
2.7.1.78 45
-
-
Homo sapiens
2.7.1.78 45
-
5 min, 92% loss of activity Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.78 6.2
-
imidazole buffer Tequatrovirus T4
2.7.1.78 7.6
-
Tris buffer Tequatrovirus T4

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.78 7.4 8 forward reaction, optimal range Tequatrovirus T4

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.78 ADP reverse reaction Tequatrovirus T4
2.7.1.78 ATP dependent on Homo sapiens
2.7.1.78 ATP dependent on Rattus norvegicus
2.7.1.78 ATP dependent on Bos taurus
2.7.1.78 ATP dependent on Mesocricetus auratus
2.7.1.78 ATP dependent on Tequatrovirus T4
2.7.1.78 ATP dependent on Escherichia phage T2
2.7.1.78 ATP dependent on Enterobacteria phage T6
2.7.1.78 CDP
-
Homo sapiens
2.7.1.78 CDP
-
Rattus norvegicus
2.7.1.78 CDP
-
Bos taurus
2.7.1.78 CDP
-
Mesocricetus auratus
2.7.1.78 CDP
-
Escherichia phage T2
2.7.1.78 CTP
-
Homo sapiens
2.7.1.78 CTP
-
Rattus norvegicus
2.7.1.78 CTP
-
Bos taurus
2.7.1.78 CTP
-
Mesocricetus auratus
2.7.1.78 CTP
-
Escherichia phage T2
2.7.1.78 dATP
-
Tequatrovirus T4
2.7.1.78 GDP
-
Homo sapiens
2.7.1.78 GDP
-
Rattus norvegicus
2.7.1.78 GDP
-
Bos taurus
2.7.1.78 GDP
-
Mesocricetus auratus
2.7.1.78 GDP
-
Escherichia phage T2
2.7.1.78 GTP
-
Homo sapiens
2.7.1.78 GTP
-
Rattus norvegicus
2.7.1.78 GTP
-
Bos taurus
2.7.1.78 GTP
-
Mesocricetus auratus
2.7.1.78 GTP
-
Escherichia phage T2
2.7.1.78 TDP
-
Tequatrovirus T4
2.7.1.78 TTP
-
Tequatrovirus T4
2.7.1.78 UDP
-
Rattus norvegicus
2.7.1.78 UDP
-
Tequatrovirus T4
2.7.1.78 UDP
-
Escherichia phage T2
2.7.1.78 UTP
-
Rattus norvegicus
2.7.1.78 UTP
-
Tequatrovirus T4
2.7.1.78 UTP
-
Escherichia phage T2