BRENDA - Enzyme Database show

Characterization of nucleoside phosphotransferase from Leishmania tropica

Shin, I.S.; Wataya, Y.; Nucleosides Nucleotides 14, 13-22 (1995)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.77
0.0057
-
2'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0059
-
2'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0059
-
Inosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0061
-
adenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0063
-
2'-deoxyguanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0064
-
guanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0066
-
3'-fluoroinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0071
-
3'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0084
-
3'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.014
-
thymidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0153
-
2'-deoxycytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0167
-
uridine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0169
-
cytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.77
cytoplasm
-
Leishmania tropica
5737
-
2.7.1.77
membrane
-
Leishmania tropica
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
40000
-
gel filtration
Leishmania tropica
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.77
Leishmania tropica
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
130
-
-
Leishmania tropica
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
642003
Leishmania tropica
5'-dAMP + 3'-dAMP + nitrophenol
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxycytidine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyguanosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 3'-deoxyadenosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 3'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 3'-fluoroinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-fluoro-IMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + adenosine
-
642003
Leishmania tropica
5'-AMP + 4-nitrophenol
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + cytidine
-
642003
Leishmania tropica
4-nitrophenol + CMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + guanosine
-
642003
Leishmania tropica
4-nitrophenol + GMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + inosine
-
642003
Leishmania tropica
4-nitrophenol + IMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + thymidine
-
642003
Leishmania tropica
4-nitrophenol + TMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + uridine
-
642003
Leishmania tropica
4-nitrophenol + 5'-UMP
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.77
0.0057
-
2'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0059
-
2'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0059
-
Inosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0061
-
adenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0063
-
2'-deoxyguanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0064
-
guanosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0066
-
3'-fluoroinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0071
-
3'-deoxyadenosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0084
-
3'-deoxyinosine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.014
-
thymidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0153
-
2'-deoxycytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0167
-
uridine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
2.7.1.77
0.0169
-
cytidine
pH 7.5, 37°C, reaction with 4-nitrophenyl phosphate
Leishmania tropica
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.1.77
cytoplasm
-
Leishmania tropica
5737
-
2.7.1.77
membrane
-
Leishmania tropica
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
40000
-
gel filtration
Leishmania tropica
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
130
-
-
Leishmania tropica
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
642003
Leishmania tropica
5'-dAMP + 3'-dAMP + nitrophenol
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxycytidine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxycytidine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyguanosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyguanosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 2'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 3'-deoxyadenosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyadenosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 3'-deoxyinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-deoxyinosine 5'-phosphate
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + 3'-fluoroinosine
-
642003
Leishmania tropica
4-nitrophenol + 3'-fluoro-IMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + adenosine
-
642003
Leishmania tropica
5'-AMP + 4-nitrophenol
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + cytidine
-
642003
Leishmania tropica
4-nitrophenol + CMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + guanosine
-
642003
Leishmania tropica
4-nitrophenol + GMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + inosine
-
642003
Leishmania tropica
4-nitrophenol + IMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + thymidine
-
642003
Leishmania tropica
4-nitrophenol + TMP
-
-
-
?
2.7.1.77
4-nitrophenyl phosphate + uridine
-
642003
Leishmania tropica
4-nitrophenol + 5'-UMP
-
-
-
?