BRENDA - Enzyme Database show

Nucleoside phosphotransferase from barley. Characterization and evidence for ping pong kinetics involving phosphoryl enzyme

Prasher, D.C.; Carr, M.C.; Ives, D.H.; Tsai, T.C.; Frey, P.A.; J. Biol. Chem. 257, 4931-4939 (1982)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.7.1.77
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
Hordeum vulgare
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
Cd2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
Cu2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
EDTA
2 mM, 85% inhibition. 95% inhibition at 10 mM
Hordeum vulgare
2.7.1.77
Hg2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
Zn2+
2 mM, 20% inhibition of transphosphorylation
Hordeum vulgare
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.77
0.087
-
deoxyadenosine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
0.412
-
deoxyguanosine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
0.89
-
deoxythymidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
2.13
-
guanosine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
3.93
-
uridine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
4.15
-
deoxycytidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
6.39
-
cytidine
pH 5.0, reaction with AMP
Hordeum vulgare
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.77
Ba2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
2.7.1.77
Co2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
2.7.1.77
Mg2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation
Hordeum vulgare
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
50000
-
2 * 50000, SDS-PAGE
Hordeum vulgare
2.7.1.77
53400
-
non-denaturing PAGE
Hordeum vulgare
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.77
Hordeum vulgare
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.77
glycoprotein
carrying a variable amount of polysaccharide
Hordeum vulgare
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.77
-
Hordeum vulgare
Reaction
EC Number
Reaction
Commentary
Organism
2.7.1.77
a nucleotide + a 2'-deoxyribonucleoside = a nucleoside + a 2'-deoxyribonucleoside 5'-phosphate
ping pong kinetics
Hordeum vulgare
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.77
seedling
-
Hordeum vulgare
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
180
-
-
Hordeum vulgare
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.77
-20°C, stable for at least 6 months
Hordeum vulgare
2.7.1.77
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641994
Hordeum vulgare
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
2.7.1.77
dimer
2 * 50000, SDS-PAGE
Hordeum vulgare
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.77
additional information
-
additional information
-
Hordeum vulgare
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.77
5.5
-
phosphatase activity
Hordeum vulgare
2.7.1.77
6.5
-
transferase activity
Hordeum vulgare
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.77
4.5
8.5
pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
Hordeum vulgare
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.1.77
withstands repeated freezing and thawing if the protein concentration is less than 0.5 mg/ml
Hordeum vulgare
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
Cd2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
Cu2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
EDTA
2 mM, 85% inhibition. 95% inhibition at 10 mM
Hordeum vulgare
2.7.1.77
Hg2+
2 mM, at least 80% inhibition of transphosphorylation
Hordeum vulgare
2.7.1.77
Zn2+
2 mM, 20% inhibition of transphosphorylation
Hordeum vulgare
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.77
0.087
-
deoxyadenosine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
0.412
-
deoxyguanosine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
0.89
-
deoxythymidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
2.13
-
guanosine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
3.93
-
uridine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
4.15
-
deoxycytidine
pH 5.0, reaction with AMP
Hordeum vulgare
2.7.1.77
6.39
-
cytidine
pH 5.0, reaction with AMP
Hordeum vulgare
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.1.77
Ba2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
2.7.1.77
Co2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement
Hordeum vulgare
2.7.1.77
Mg2+
2 mM, activity with AMP and deoxythymidine is enhanced about 50%, no absolute requirement; 2 mM, with 0.5 mM AMP and deoxythymidine, 50% stimulation
Hordeum vulgare
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
50000
-
2 * 50000, SDS-PAGE
Hordeum vulgare
2.7.1.77
53400
-
non-denaturing PAGE
Hordeum vulgare
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.7.1.77
glycoprotein
carrying a variable amount of polysaccharide
Hordeum vulgare
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.77
-
Hordeum vulgare
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.77
seedling
-
Hordeum vulgare
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
180
-
-
Hordeum vulgare
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.77
-20°C, stable for at least 6 months
Hordeum vulgare
2.7.1.77
0°C, 0.01% Triton X-100 or 0.25 M sodium acetate, pH 5, 44 h, phosphotransferase activity decreases 20% and 50% respectively
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
4-nitrophenyl phosphate + 2'-deoxyadenosine
-
641994
Hordeum vulgare
4-nitrophenol + 2'-deoxyadenosine 5'-phosphate
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.7.1.77
dimer
2 * 50000, SDS-PAGE
Hordeum vulgare
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.7.1.77
additional information
-
additional information
-
Hordeum vulgare
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.77
5.5
-
phosphatase activity
Hordeum vulgare
2.7.1.77
6.5
-
transferase activity
Hordeum vulgare
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.7.1.77
4.5
8.5
pH 4.5: about 65% of maximal activity, pH 8.5: about 50% of maximal activity
Hordeum vulgare