BRENDA - Enzyme Database show

Nucleoside phosphotransferase from carrot

Brunngraber, E.F.; Methods Enzymol. 51, 387-394 (1978)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.77
NaF
0.25 M, enhances activity by about 45%
Daucus carota
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
-
Daucus carota
2.7.1.77
cysteine
-
Daucus carota
2.7.1.77
EDTA
-
Daucus carota
2.7.1.77
Fe2+
-
Daucus carota
2.7.1.77
Fe3+
-
Daucus carota
2.7.1.77
glutathione
-
Daucus carota
2.7.1.77
Iodine
-
Daucus carota
2.7.1.77
Mn2+
-
Daucus carota
2.7.1.77
NaF
0.25 M, phosphatase is inhibited by less than 10%
Daucus carota
2.7.1.77
Pb2+
-
Daucus carota
2.7.1.77
uridine
inhibitor of phosphatase reaction
Daucus carota
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.77
3.5
-
Phenylphosphate
phosphotransferase reaction with uridine
Daucus carota
2.7.1.77
3.5
-
uridine
phosphotransferase reaction with phenyl phosphate
Daucus carota
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
44000
-
gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation
Daucus carota
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.1.77
Daucus carota
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.1.77
-
Daucus carota
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.77
root
-
Daucus carota
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
32
-
-
Daucus carota
Storage Stability
EC Number
Storage Stability
Organism
2.7.1.77
-20°C, stable for at least 1 months
Daucus carota
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
additional information
-
641991
Daucus carota
?
-
-
-
-
2.7.1.77
phenyl phosphate + adenosine
-
641991
Daucus carota
AMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + cytidine
-
641991
Daucus carota
CMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyadenosine
-
641991
Daucus carota
dAMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxycytidine
-
641991
Daucus carota
dCMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyguanosine
-
641991
Daucus carota
dGMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyuridine
-
641991
Daucus carota
dUMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + guanosine
-
641991
Daucus carota
GMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + thymidine
-
641991
Daucus carota
TMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + uridine
-
641991
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.77
5
-
-
Daucus carota
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.1.77
9.5
-
without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h
Daucus carota
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.77
Daucus carota
intact enzyme
-
4.1
2.7.1.77
Daucus carota
subunit A
4.7
4.6
2.7.1.77
Daucus carota
subunit B
-
5.1
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.1.77
NaF
0.25 M, enhances activity by about 45%
Daucus carota
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.7.1.77
Ca2+
-
Daucus carota
2.7.1.77
cysteine
-
Daucus carota
2.7.1.77
EDTA
-
Daucus carota
2.7.1.77
Fe2+
-
Daucus carota
2.7.1.77
Fe3+
-
Daucus carota
2.7.1.77
glutathione
-
Daucus carota
2.7.1.77
Iodine
-
Daucus carota
2.7.1.77
Mn2+
-
Daucus carota
2.7.1.77
NaF
0.25 M, phosphatase is inhibited by less than 10%
Daucus carota
2.7.1.77
Pb2+
-
Daucus carota
2.7.1.77
uridine
inhibitor of phosphatase reaction
Daucus carota
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.1.77
3.5
-
Phenylphosphate
phosphotransferase reaction with uridine
Daucus carota
2.7.1.77
3.5
-
uridine
phosphotransferase reaction with phenyl phosphate
Daucus carota
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.1.77
44000
-
gel filtration, equilibrium sedimentation, sucrose density gradient centrifugation
Daucus carota
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.1.77
-
Daucus carota
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.1.77
root
-
Daucus carota
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.1.77
32
-
-
Daucus carota
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.7.1.77
-20°C, stable for at least 1 months
Daucus carota
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.1.77
additional information
-
641991
Daucus carota
?
-
-
-
-
2.7.1.77
phenyl phosphate + adenosine
-
641991
Daucus carota
AMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + cytidine
-
641991
Daucus carota
CMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyadenosine
-
641991
Daucus carota
dAMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxycytidine
-
641991
Daucus carota
dCMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyguanosine
-
641991
Daucus carota
dGMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + deoxyuridine
-
641991
Daucus carota
dUMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + guanosine
-
641991
Daucus carota
GMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + thymidine
-
641991
Daucus carota
TMP + phenol
-
-
-
?
2.7.1.77
phenyl phosphate + uridine
-
641991
Daucus carota
5'-UMP + phenol
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.1.77
5
-
-
Daucus carota
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.7.1.77
9.5
-
without protecting agent, about one-third of the initial transfer activity is retained whereas the hydrolase function is almost entirely lost after 22 h
Daucus carota
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
2.7.1.77
Daucus carota
intact enzyme
-
4.1
2.7.1.77
Daucus carota
subunit A
4.7
4.6
2.7.1.77
Daucus carota
subunit B
-
5.1